Recombinant Mouse Apolipoprotein E (HEK293, C-6His)

Biological Activity

Apolipoprotein E (Apo-E) is a member of the apolipoprotein A1/A4/E family. APOE may function in mediating the binding, internalization, and catabolism of lipoprotein particles. Apolipoprotein E (Apo-E) can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. 

Recombinant Mouse Apolipoprotein E expressed the target gene encoding Glu19-Gln311 with a 6His tag at the C-terminus. 

Accession: P08226

Endotoxin < 1 EU/µg

Apparent Molecular Weight: 30-40 KDa, reducing conditions

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Chemical Information

Form	Lyophilized powder
Solubility (25°C)	Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
Storage	Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.

References

[1] Claudia Marino, et al. Alzheimers Dement. APOE Christchurch-mimetic therapeutic antibody reduces APOE-mediated toxicity and tau phosphorylation

[2] Eu Jeong Ku, et al. Int J Mol Sci. The Anti-Atherosclerosis Effect of Anakinra, a Recombinant Human Interleukin-1 Receptor Antagonist, in Apolipoprotein E Knockout Mice

[3] Martin Maeng Bjørklund, et al. Circ Res. Induction of atherosclerosis in mice and hamsters without germline genetic engineering

[4] Akin Akinc, et al. Mol Ther. Targeted delivery of RNAi therapeutics with endogenous and exogenous ligand-based mechanisms

[5] D M Holtzman, et al. J Clin Invest. Expression of human apolipoprotein E reduces amyloid-beta deposition in a mouse model of Alzheimer's disease