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> Recombinant Human Interleukin-13 (IL-13) Protein

Recombinant Human Interleukin-13 (IL-13) Protein

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Referencia 230-30127-10

embalaje : 10ug

Marca : RayBiotech

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Recombinant Human Interleukin-13 (IL-13) ProteinStimulating cell growth significantly. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.

Recombinant Human Interleukin-13 (IL-13) Protein

Recombinant Human Interleukin-13 (IL-13) Protein

Recombinant human interleukin-13 (IL-13) protein, derived from the transfected human HEK293 cells.
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Product Description

Specifications

Size10 µg, 50 µg, 100 µg
SpeciesHuman
Accession Number
P35225
Gene Symbols
IL13
Protein Name / Synonyms
IL-13, Interleukin-13
Expressed Region
Gly35-Asn146
Expression SystemHEK293 cells
TagC-terminal his-tag
Purity
>95%
Purity Determined BySDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)
Recombinant protein product has a calculated molecular mass of 13 kDa. Due to the abundant glycosylation, it migrates as approximately 13-35 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Format
Lyophilized powder
Formulation
Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4)
Concentration (lot specific)
Determined by BCA protein assay
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Research AreaInflammation
Shipping TypeAmbient temperature
Storage-20°C
SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Activity
Stimulating cell growth significantly. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.

Stability & Storage

Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

Custom Labeling Request

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

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