Recombinant Human COX-2 Protein (Baculovirus-Insect, C-His)

Biological Activity

COX-2 is s component of Prostaglandin-endoperoxide synthase (PTGS). It is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. COX-2 is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. It mediates the formation of prostaglandins from arachidonate and may have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.

The recombinant human COX-2 consists of 597 amino acids and predicts a molecular mass of 68.5 kDa. A DNA sequence encoding the human COX-2 (Met 1-Leu 604) was fused with a polyhistidine tag at the C-terminus.

Endotoxin < 1 EU/µg

Apparent Molecular Weight: 66 kDa, reducing conditions

Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.0.

Chemical Information

Form	Lyophilized powder
Storage	Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.

References

[1] Chien-Chung Yang, et al. Oxid Med Cell Longev. Sphingosine 1-Phosphate-Upregulated COX-2/PGE2 System Contributes to Human Cardiac Fibroblast Apoptosis: Involvement of MMP-9-Dependent Transactivation of EGFR Cascade

[2] Ming-Yi Ho, et al. Oncotarget. Recombinant viral capsid protein VP1 suppresses lung cancer metastasis by inhibiting COX-2/PGE2 and MIG-7

[3] Cassie Lin, et al. Carcinogenesis. Inducible COX-2-dependent apoptosis in human ovarian cancer cells

[4] James K Gierse. Methods Mol Biol. Purification of recombinant human COX-1 and COX-2

[5] Yun S Lee, et al. Mol Hum Reprod. The role of CCAAT/enhancer-binding protein beta in the transcriptional regulation of COX-2 in human amnion