Apolipoprotein E (ApoE) is a 299 amino acid protein, produced by the liver and circulating macrophages, that is a constituent of every plasma lipoprotein except the smallest low density lipoproteins (LDL). It is a key player in the recycling and redistribution of lipids and cholesterol. ApoE is a ligand with a high affinity for low density lipoprotein receptors (LDLR). It regulates the activity of enzymes that metabolize lipids and also makes lipids soluble. Mice and humans that lack ApoE cannot remove excess lipoproteins from the plasma and have an increased risk of atherosclerosis. Defective binding of ApoE to its receptors will lead to accumulation of cholesterolrich lipoprotein particles in the plasma; this is the cause of type III hyperlipoproteinemia. There are three main isoforms of ApoE all products of alleles at a single gene locus: E2 (Cys112, Cys158), E3 (C112, Arg158), and E4 (Arg112, Arg158). ApoE3 is the most common isoform and is present in 4090% of the population.

Data not yet available.

HHHHHHKVEQ AVETEPEPEL RQQTEWQSGQ RWELALGRFW DYLRWVQTLS EQVQEELLSS QVTQELRALM DETMKELKAY KSELEEQLTP VAEETRARLS KELQAAQARL GADMEDVCGR LVQYRGEVQA MLGQSTEELR VRLASHLRKL RKRLLRDADD LQKRLAVYQA GAREGAERGL SAIRERLGPL VEQGRVRAAT VGSLAGQPLQ ERAQAWGERL RARMEEMGSR TRDRLDEVKE QVAEVRAKLE EQAQQIRLQA EAFQARLKSW FEPLVEDMQR QWAGLVEKVQ AAVGTSAAPV PSDNH

Met

Lyophilized

The predicted molecular weight of Recombinant Human Apolipoprotein E3 is 35.2 kDa.

This lyophilized protein is 0.2 µm sterile filtered and lyophilized from 20 mM Sodium Phosphate, Ph 7.8 + 0.5 mM DTT.

The lyophilized protein should be stored desiccated at 20°C. The reconstituted protein can be stored for at least one week at 4°C. For longterm storage of the reconstituted protein, aliquot into working volumes and store at 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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1. Sennlaub, F. et al. (2015) J Neurosci. 35(40):13568–76. PubMed
2. Yang, H. et al. (2012) PLoS One. 7(9):e44430. PubMed