Rat TNF Alpha Protein (Recombinant)
Referencia LS-G408-20
embalaje : 20ug
Marca : LSBio
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Teléfono : +1 850 650 7790
Overview
Description:
TNF Alpha Protein LS-G408 is a Recombinant Rat TNF Alpha produced in E. coli. It is biologically active and is low in endotoxin; Less than 0.1 ng/µg of protein (less than 1EU/µg). For Research Use Only
Specifications
Type
Recombinant Protein
Target
TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Species
Rat
Modifications
Unmodified
Conjugations
Unconjugated
Predicted Molecular Weight
17.3 kDa
AA Sequence
MLRSSSQNSSDKPVAHVVANHQAEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLIYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVSLLSAIKSPCPKDTPEGAELKPWYEPMYLGGVFQLEKGDLLSAEVNLPKYLDITESGQVYFGVIAL Expression System
E. coli
Source Species
E. coli
Purification
Greater than 98% by SDS-PAGE and HPLC
Bio-Activity
The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is <=0.05 ng/ml, corresponding to a specific activity of >=2 x 10^7 units/mg.
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Sterile filtered, lyophilized from 0.5X PBS, pH 7.1
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee About TNF Alpha
P01375 NM_000594 NP_000585.2
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
tech@quimigen.pt