Rat secretory immunoglobulin A,sIgA ELISA Kit
Referencia CSB-E08412r-96T
embalaje : 96T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
secretory immunoglobulin A,sIgA
Abbreviation
sIgA
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.78 ng/mL-50 ng/mL
Sensitivity
0.195 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 102 | ||||
| Range % | 97-106 | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 86-96 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:800 | Average % | 88 | ||||
| Range % | 84-92 | |||||
Recovery
| The recovery of rat sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Rat sIgA ELISA Kit was designed for the quantitative measurement of Rat sIgA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .
Citations
- X Shen,SSRN,2023
- MJ Kim,Laboratory animal research,2022
- Impact of winter savory extract (Satureja montana L.) on biochemical parameters in serum and oxidative status of liver with application of the principal component analysis in extraction solvent selection B Milijašević,European review for medical and pharmacological sciences,2022
- S Sun,Molecular Nutrition & Food Research,2021
- Jian Zhou.et al,British Journal of Nutrition,2018
- Kuzubova NA.et al,Bull Exp Biol Med.,2015
Customer Reviews and Q&A
Average Rating:
Sample type: Serum
Sample species: Rat
Sample dilution: No dilution
Review: I used CSB-E08412R to detect the content of HMGB1 in colon and lung tissue homogenates. The product is convenient and fast, with high sensitivity. The experimental results are reliable, the detection results are reliable, and the trend is obvious. It is recommended to use.
By Anonymous