Rat Myoglobin,MYO/MB ELISA Kit
Referencia CSB-E08049r-96T
embalaje : 96T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
myoglobin
Associated names
Mb ELISA Kit; Myoglobin ELISA Kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 ng/mL-1000 ng/mL
Sensitivity
3.9 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MYO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 83-96 | |||||
Recovery
| The recovery of rat MYO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 88-99 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Rat Myoglobin(MB) ELISA Kit was designed for the quantitative measurement of Rat Myoglobin(MB) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL .
Citations
- Revolutionizing hyperlipidemia treatment: Nanoencapsulated CoQ10 and selenium combat simvastatin-induced myopathy and insulin resistance in rats HB Abo-zalam,/,2023
- F Yu,Toxicon,2023
- J Han,Toxicon,2022
- Chen X, et al,Thrombosis and Haemostasis,2019
- El-Ganainy SO.et al,Toxicology.,2016
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Gene References into Functions
- In rhabdomyolytic rats, the level of heme and free iron in cytoplasm and mitochondria of kidney cells is remarkably increased while inhibition of proteolysis results in further elevation of myoglobin content in the renal tissue.
- After reperfusion, there was no significant increase in dialysate myoglobin concentration. MDL-28170 did not affect dialysate myoglobin concentration during occlusion or after reperfusion.
- Amphetamine-induced serum myoglobin release is a potential biomarker and possibly a factor in AMPH-induced toxicity processes.
- bradykinin-induced NO does not interact significantly with cellular Mb to produce an NMR-detectable quantity of metMb in the perfused rat myocardium
- determination of myoglobin concentration and succinate dehydrogenase activity in serial sections indicate that myoglobin can lead to a substantial reduction (18-60%) of the extracellular oxygen tension required to prevent an anoxic core in muscle cells
- Mb-facilitated diffusion has a higher poise in skeletal muscle than in myocardium.
- Moreover, the similar Q10 for the DMb of solution versus cell Mb suggests that any temperature-dependent alteration of the postulated cell matrix does not significantly affect protein mobility.
- The nitrite reductase activity of deoxymyoglobin reduces nitrite approximately 36 times faster than deoxyhemoglobin because of its lower heme redox potential.
- intermittent hypobaric hypoxia decreased myocardial citrate synthase activity and myoglobin and decreased myocardial lactate dehydrogenase activity
- Data suggest that rhabdomyolysis-induced kidney damage involves direct interaction of myoglobin with mitochondria possibly resulting in iron ions release from myoglobin's heme, which promotes the peroxidation of mitochondrial membranes.
Protein Families
Globin family
Database Links
KEGG:
STRING:
UniGene: