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Rat Leptin ELISA Kit
Rat Leptin ELISA Kit
RayBio® Rat Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Rat |
| Sensitivity | 30 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 30 pg/ml - 8000 pg/ml |
| Specificity | The Leptin ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, L-Selectin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 3 fold |
| Accession Number | Q9WVB7 |
| Gene Id | 113917 |
| Gene Symbols | LENEP, LEP503 |
| Protein Name / Synonyms | Lens epithelial cell protein LEP503 |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Rat Leptin qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Rat Leptin ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Leptin (Lenep), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Perivascular adipose tissue from female rats fed a high-fat diet impaired mesenteric artery vasodilation
- Species:
- Rat
- Sample Type:
- Plasma (female rats on high-fat diet)
- Year:
- 2026
- Journal Name:
- Physiological Reports
- PubMed ID:
- 41603485
Chromium and formoterol therapy for obesity-induced asthma in rats
- Species:
- Rat
- Sample Type:
- Serum (obesity-induced asthma)
- Year:
- 2025
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 40242447
Intergenerational effects of cafeteria diet-induced obesity on metabolic and reproductive outcome in rats
- Species:
- Rat
- Sample Type:
- Serum (F0 and F1 generations)
- Year:
- 2025
- Journal Name:
- Scientific Reports
- PubMed ID:
- 40425650
Obesity Promotes Renal Inflammation and Fibrosis Independent of Sex in SS Leptin Receptor Mutant (SSLepR) Rats
- Species:
- Rat
- Sample Type:
- Plasma (SS and SSLepR mutant rats, both sexes)
- Year:
- 2025
- Journal Name:
- Biomedicines
- PubMed ID:
- 41463113
Catechins prevent obesity-induced kidney damage by modulating PPARγ/CD36 pathway and gut-kidney axis in rats
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- LIFE SCIENCES
The Role of Lymph-Adipose Crosstalk in Alcohol-Induced Perilymphatic Adipose Tissue Dysfunction
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (PLAT, SFAT tissue homogenates)
- Year:
- 2024
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 39409139
Vinpocetine and Lactobacillus improve fatty liver in rats: role of adiponectin and gut microbiome
- Species:
- Rat
- Sample Type:
- Serum (fatty liver rats, vinpocetine/Lactobacillus)
- Year:
- 2024
- Journal Name:
- Amb Express
- PubMed ID:
- 39095672
Quinoa Ameliorates Hepatic Steatosis, Oxidative Stress, Inflammation and Regulates the Gut Microbiota in Nonalcoholic Fatty Liver Disease Rats
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Foods (Basel, Switzerland)
Xiaoyaosan ameliorates depressive-like behavior and susceptibility to glucose intolerance in rat: involvement of LepR-STAT3/PI3K pathway in hypothalamic arcuate nucleus
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- BMC complementary medicine and therapies
Protective Effects of a Discontinuous Treatment with Alpha-Lipoic Acid in Obesity-Related Heart Failure with Preserved Ejection Fraction, in Rats
- Species:
- Rat
- Sample Type:
- Plasma (obesity-related HFpEF ± alpha-lipoic acid)
- Year:
- 2020
- Journal Name:
- Antioxidants
- PubMed ID:
- 33142857
