Rat IgM HEAVY CHAIN Antibody
Referencia OASA04178
embalaje : 0.25mg
Marca : Aviva Systems Biology
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Rat IgM HEAVY CHAIN Antibody (OASA04178)
| Datasheets/Manuals | Printable datasheet for Rat IgM HEAVY CHAIN Antibody (OASA04178) |
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| Predicted Species Reactivity | Rat |
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| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | MARM-4 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | ELISA |
| Additional Information | Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line. |
| :: | Preparation: Purified IgG prepared by affinity chromatography Preservative Stabilisers: 0.09% - Sodium Azide |
| :: | Approx Protein Conc: IgG concentration 1.0 mg/ml |
| Reconstitution and Storage | -20°C |
| Immunogen | IR202 rat IgM myeloma protein |
| Purification | Purified IgG prepared by affinity chromatography from tissue culture supernatant |
| Predicted Homology Based on Immunogen Sequence | Rat |
| Concentration | 1 mg/ml |
| Specificity | IgM |
| Application Info | ELISA : This antibody may be used as a coating antibody in a sandwich ELISA in combination with clone MARK-1/MARL-15 (OASA0579996P) as detection reagent and purified rat IgM (OPSA10730). |
| Protocol Information | Citation: 1: Coombe DR, Watt SM, Parish CR. Mac-1 (CD11b/CD18) and CD45 mediate theadhesion of hematopoietic progenitor cells to stromal cell elements via recognition of stromal heparan sulfate. Blood. 1994 Aug 1;84(3):739-52. PubMed PMID: 8043862. Species: A4 Cells Experiment Name: Flow cytometry Experiment Background: The extent of MoAb binding to A4 cells was measured by the geometric mean fluorescence intensity (fluorescence intensity) determined by flow cytometry. Experimental Steps: 1. 3T3 or RF208 cells from 72-hour, subconfluent monolayers were released from the flask by incubating at 37C in 10 mmol/L EDTA/PBS containing 0.5% Ficoll (Pharmacia LKB, Uppsala, Sweden). 2. The cells were washed in RPMI/BSA and resuspended to 4 X 106/mL. 3. Twenty microliters of cells was added to 20 uL of biotinylated A4 cell lysate diluted 1/5 in RPMI/BSA in the wells of a V-bottomed 96-well plate (Costar, Cambridge, MA) and incubated on ice for 30 minutes. 4. The cells were washed with 150 uL/well of ice-cold RPMI/BSA, the plate centrifuged at 200g for 1 minute, and the supernatant removed. 5. After repeating the wash three times, the cells were resuspended. To detect binding of biotinylated material, 20 uL of FITC-conjugated streptavidin diluted 1/15 in RPMI/BSA was added and the plate incubated on ice for 30 minutes. 6. The cells were then washed, fixed with 200 uL/well of 2% paraformaldehyde/PBS, and analyzed in a FACScan flow cytometer (Becton Dickinson, San Jose, CA). Viable cells were gated on the basis of forward- and side-scatter parameters and analyzed for fluorescein fluorescence using the Lysis II system (Becton Dickinson). Geometric mean fluorescence intensity was the measure of staining. The relative intensity of staining was determined by comparison to cells reacted with a 1/5 dilution of lysis buffer or with RPMI/BSA and stained with FITC-streptavidin (negative control). Experiments were performed in triplicate. For inhibition of binding assays, 20 uL of biotinylated lysate (diluted 1/5 in RPMVBSA) was preincubated on ice for 30 minutes with 20 uL of GAG diluted as indicated in RPMI/BSA, then 3T3 cells added and the assay performed. Other Reagents Used: HEPES Number Of Protocols: 1 |
| Reference | 1. Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c Eur. J. Immunol. 1974, 4: 44-48. 2. Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat Immunol. 121: 2077-2082. 3. Khalifeh, M.S. et al. (2010) Investigation ofthe role of tumour necrosis factor-{alpha}, interleukin-1{beta}, interleukin-10, nitric oxide and rheumatoid factor-immunoglobulin M in a rat model of arthritis. |
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| Protein Name | Ig mu chain C region |
| Description of Target | MOUSE ANTI RAT IgM HEAVY CHAIN |
| Uniprot ID | P01871 |
| Protein Size (# AA) | 115 |
