Rat IgG (H+L), Mouse ads Antibody : PE

Referencia OASB01937

embalaje : 0.25mg

Marca : Aviva Systems Biology

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Goat Anti-Rat IgG(H+L) Antibody - PE Conjugated (OASB01937)

Datasheets/ManualsPrintable datasheet for Goat Anti-Rat IgG(H+L) Antibody - PE Conjugated (OASB01937)
Product Info
Tested Species ReactivityRat
Predicted Species ReactivityRat
ClonalityPolyclonal
IsotypeIgG
HostGoat
ConjugationR-PE
ApplicationFLISA, FC
Additional InformationDescription: Pepsin digest of Goat Anti-Rat IgG(H+L), Mouse ads - Pooled antisera from goats hyperimmunized with rat IgG; purified by affinity chromatography on pooled rat IgG covalently linked to agarose; reacts with the heavy and light chains of rat IgG and the light chains of rat IgM; cross-adsorbed against mouse immunoglobulins and pooled sera for minimal reactivity
Reconstitution and StorageStore at 2-8C
ImmunogenRat IgG
PurificationGel filtration of pepsin- digested antibody.
Concentration0.25 mg/mL
SpecificityIgG(H+L)
CharacterizationTo ensure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometryfor conformance to characteristics of a standard reference reagent.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionImmunofluorescence:
FLISA:
ELISA:
UNLB, FITC, and BIOT conjugates
R-PE, R-PE-TXRD, SPRD and R-PE-CY5.5
conjugates
TXRD conjugates
BIOT conjugate
<= 1 ug/106 cells
<= 0.1 ug/106 cells
1:100 – 1:400
1:5,000-1:20,000
Application InfoDirect and indirect immunofluorescent staining, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting, Immunohistochemistry
Other Applications DataSince applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
Storage- The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) and Texas Red (TXRD) conjugates are supplied as 0.5 mg in 1.0 mLPBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD), R- phycoerythrin- Texas Red (R- PE- TXRD), and R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze!
- The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C
- Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
Cross AbsorptionPooled mouse sera and pooled mouse plasmacytoma/hybridoma proteins.
Gene Full NameIg gamma-1 chain C region