Rat IgG (H+L) Antibody : FITC

Referencia OASB02087

embalaje : 0.5mg

Marca : Aviva Systems Biology

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Rabbit Anti-Rat IgG(H+L) Antibody - FITC Conjugated (OASB02087)

Datasheets/ManualsPrintable datasheet for Rabbit Anti-Rat IgG(H+L) Antibody - FITC Conjugated (OASB02087)
Product Info
Tested Species ReactivityRat
Predicted Species ReactivityRat
ClonalityPolyclonal
IsotypeIgG
HostRabbit
ConjugationFITC
ApplicationFLISA, IHC-F, IHC-P, ICC, WB
Additional InformationDescription: Pepsin digest of Rabbit Anti-Rat IgG(H+L) - Pooled antisera from rabbits hyperimmunized with rat IgG; purified by affinity chromatography on rat IgG covalently linked to agarose; reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Reconstitution and StorageStore at 2-8C
ImmunogenRat IgG
PurificationAffinity chromatography on rat IgG covalently coupled to agarose. Gel filtrationchromatography of pepsin digested antibody.
Concentration0.5 mg/mL
SpecificityIgG(H+L)
CharacterizationTo ensure lot- to- lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flowcytometry for conformance to characteristics of a standard reference reagent.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionImmunofluorescence:
ELISA:
FITC and TRITC conjugates
AP conjugate
HRP conjugate
<= 1 ug/106 cells
1:2,000-1:4,000
1:4,000-1:8,000
Application InfoDirect immunofluorescent staining, Enzyme-Linked-Immunosorbent-Assay (ELISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry
Other Applications DataSince applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
Storage- The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) and rhodamine (TRITC) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3
Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
Cross AbsorptionNA
Gene Full NameIg gamma-1 chain C region