Rat IgA HEAVY CHAIN Antibody
Referencia OASA04154
embalaje : 0.25mg
Marca : Aviva Systems Biology
Contact local distributor :
Teléfono : +1 850 650 7790
Rat IgA HEAVY CHAIN Antibody (OASA04154)
| Datasheets/Manuals | Printable datasheet for Rat IgA HEAVY CHAIN Antibody (OASA04154) |
|---|
| Predicted Species Reactivity | Rat |
|---|---|
| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | MARA-1 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | ELISA|IF|IHC-Fr |
| Additional Information | Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
| :: | Preparation: Purified IgG prepared by affinity chromatography Preservative Stabilisers: 0.09% - Sodium Azide |
| :: | Approx Protein Conc: IgG concentration 1 mg/ml Buffer Solutions: Phosphate buffered saline pH7.4 |
| Reconstitution and Storage | -20°C |
| Immunogen | Purified IR1060 IgA rat myeloma protein. |
| Purification | Purified IgG prepared by affinity chromatography from tissue culture supernatant |
| Predicted Homology Based on Immunogen Sequence | Rat |
| Concentration | 1 mg/ml |
| Specificity | IgA |
| Application Info | ELISA : This antibody may be used as a coating antibody in a sandwich ELISA in combination with clone MARK-1/MARL-15 (OASA0579996P) as detection reagent and purified rat IgA (OPSA10723). |
| Protocol Information | Citation: 1: Ravindranath RM, Mondino BJ, Adamu SA, Pitchekian-Halabi H, Hasan SA, Glasgow BJ. Immunopathologic features of Staphylococcus aureus endophthalmitis in therat. Invest Ophthalmol Vis Sci. 1995 Nov;36(12):2482-91. PubMed PMID: 7591638. Species: Rat Experiment Name: Enzyme-linked immunosorbent assay Experiment Background: To study the clinical, histopathologic, and immunologic responses to Staphylococcus aureus endophthalmitis in rats Experimental Steps: Experimental Lewis rats received an intravitreal injection of viable S. aureus (65 organisms), and control rats received sterile saline. The clinical scores, cellular infiltrate, delayed hypersensitivity reaction in skin tests, and serum and vitreous enzyme-linked immunosorbent assay titers of immunoglobulin (Ig) M, IgG, and IgA to ribitol teichoic acid (RTA), the major antigenic determinant of 5. aureus cell wall, were measured and compared on days 3, 7, 10, 14, 21, and 30. The differences were statistically assessed using Mann-Whitney nonparametric <-tests and analysis of variance. Number Of Protocols: 1 |
| Reference | 1. Kohler, G. & Milstein, C. (1975) Continuous cultures of fused cells secreting antibody of predefined specificity Nature, 256: 495-497. 2. Schulman, M., Wilde, C.D. & Kohler, G. (1978) A better cell line for making hybridomas secreting specific antibodies Nature, 276: 269-270. 3. Bazin, H., Beckers, A. & Querinjean, P. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c Eur.J. Immunol. 4: 44-48. 4. Bazin, H., Beckers, A., Urbain-Vansanten, G., Pauwels, R., Bruyns, C., Tilkin, A.F., Platteau, B. & Urbain, J.J. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat Immunol 121: 2077-2082. 5. Bazin, H., et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum J. Immunol. Methods 71: 9-16. 6. Grabar and Williams (1953) Method permitting the combined study of the electrophoretic and the immunochemical properties of protein mixtures; application to blood serum. Biochem. Biophys. Acta 10: 193. |
|---|---|
| Protein Name | Ig alpha-1 chain C region |
| Description of Target | MOUSE ANTI RAT IgA HEAVY CHAIN |
| Uniprot ID | P01876 |
| Protein Size (# AA) | 131 |
