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Rat CRP ELISA Kit
Rat CRP ELISA Kit
RayBio® Rat CRP (C-Reactive Protein) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Rat |
| Sensitivity | 200 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.2 ng/ml - 60 ng/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 20,000 - 200,000 fold |
| Accession Number | P22282 |
| Gene Id | 25030 |
| Gene Symbols | ANDPRO, CRP1 |
| Protein Name / Synonyms | Cystatin-related protein 1 (CRP-1) (Androgen-regulated 20 kDa protein) (Prostatic 22 kDa glycoprotein P22K16/P22K20) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Rat CRP qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Rat CRP ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified CRP (C-Reactive Protein), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Docosahexaenoic Acid Consumption Early in Life Improves Immune Dysfunction in High-Fat Diet-Fed Wistar Rats in a Sex-Specific Manner
- Species:
- Rat
- Sample Type:
- Plasma (high-fat diet, DHA supplementation)
- Year:
- 2026
- Journal Name:
- Molecular Nutrition & Food Research
- PubMed ID:
- 41566742
Single high-dose intravenous injection of Wharton's jelly-derived mesenchymal stem cell exerts protective effects in a rat model of metabolic syndrome
- Species:
- Rat
- Sample Type:
- Serum (metabolic syndrome rats)
- Year:
- 2024
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 38835014
Modulation of NLRP3 Inflammasome Activation by QYHT Decoction: Implications for the Treatment of Erectile Dysfunction in Hyperuricemia
- Species:
- Rat
- Sample Type:
- Serum (HUA-induced ED SD rats)
- Year:
- 2025
- Journal Name:
- American journal of men's health
- PubMed ID:
- 40007058
Cryopreserved nanostructured fibrin-agarose hydrogels are efficient and safe hemostatic agents
- Species:
- Rat
- Sample Type:
- Whole Blood (blood samples EDTA)
- Year:
- 2024
- Journal Name:
- Scientific Reports
- PubMed ID:
- 39169092
Linoleyl acetate and mandenol alleviate HUA-induced ED via NLRP3 inflammasome and JAK2/STAT3 signalling conduction in rats
- Species:
- Rat
- Sample Type:
- Serum (hyperuricemia-induced ED rats)
- Year:
- 2024
- Journal Name:
- Journal of Cellular and Molecular Medicine
- PubMed ID:
- 39245800
Chondroprotective Effects of Grapefruit (Citrus paradisi Macfad.) Juice in a Complete Freund’s Adjuvant Rat Model of Knee Osteoarthritis
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Nutrients
In vivo efficacy & phantom imaging connote the theranostic potential of a drug-loaded lipid nanobubble
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Journal of Drug Delivery Science and Technology
Anti-arthritic activity of ferulic acid in complete Freund's adjuvant (CFA)-induced arthritis in rats: JAK2 inhibition
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2020
The Effect of Different Water Extracts from Platycodon grandiflorum on Selected Factors Associated with Pathogenesis of Chronic Bronchitis in Rats
- Species:
- Rat
- Sample Type:
- Serum (chronic bronchitis model)
- Year:
- 2020
- Journal Name:
- Molecules
- PubMed ID:
- 33138217
Adrenomedullin Attenuates Inflammation in White Adipose Tissue of Obese Rats Through Receptor-Mediated PKA Pathway
- Species:
- Rat
- Sample Type:
- Plasma
- Year:
- 2020
- Journal Name:
- Obesity
