- A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat COMP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled COMP antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
Rat cartilage oligomeric matrix protein,COMP ELISA Kit
Referencia CSB-E13833r-96T
embalaje : 96T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
cartilage oligomeric matrix protein
Associated names
CompCartilage oligomeric matrix protein ELISA kit; COMP ELISA kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
78 pg/ml - 5000 pg/ml
Sensitivity
19.5 pg/ml
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | |||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||
Three samples of known concentration were tested in twenty assays to assess. | |||||
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat COMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 92 | ||||
Range % | 86-107 | |||||
1:2 | Average % | 97 | ||||
Range % | 91-105 | |||||
1:4 | Average % | 98 | ||||
Range % | 92-104 | |||||
1:8 | Average % | 100 | ||||
Range % | 95-105 | |||||
Recovery
The recovery of rat COMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 96 | 91-101 | ||||
EDTA plasma (n=4) | 93 | 90-96 | ||||
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ||||||
pg/ml | OD1 | OD2 | Average | Corrected | ||
5000 | 2.639 | 2.699 | 2.669 | 2.501 | ||
2500 | 2.151 | 2.163 | 2.157 | 1.989 | ||
1250 | 1.505 | 1.542 | 1.524 | 1.356 | ||
625 | 1.062 | 1.098 | 1.080 | 0.912 | ||
312 | 0.637 | 0.689 | 0.663 | 0.495 | ||
156 | 0.392 | 0.399 | 0.396 | 0.228 | ||
78 | 0.332 | 0.347 | 0.340 | 0.172 | ||
0 | 0.168 | 0.167 | 0.168 |
| ||
Materials provided
Materials not provided
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
The product CSB-E13833r is a ready-to-use microwell, strip plate ELISA Kit for quantitative analysis of rat cartilage oligomeric matrix protein (COMP) in biological samples, including serum, plasma, or tissue homogenates. This kit has undergone rigorous quality tests in multiple parameters, including specificity, sensitivity, precision, and lot-to-lot consistency. The detection mechanism of this kit is based on the Sandwich-ELISA technique. COMP in the samples or standards are sandwiched between pre-coated COMP antibody and Biotin-conjugated COMP antibody. The COMP/pre-coated COMP...
Citations
- Y Gou,Journal of orthopaedic surgery and research,2024
- MM Saber,Biomedicine & pharmacotherapy,2023
- W Xie,Phytomedicine,2023
- A Fitriyah,Chemosphere,2022
- SC Lin,Evidence-Based Complementary and Alternative Medicine,2021
- G Yang,Drug Design, Development and Therapy,2020
- Peng K T, et al,Clinical Science,2019
- Gou Y, et al,Bone,2019
- Yu Gou.et al,Med Sci Monit.,2018
- Aborehab NM.et al,BMC Complement Altern Med,2017
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for...
Gene References into Functions
- COMP could normally have a protective role against PASMC phenotype switching
- these findings revealed the essential role of COMP in retarding the development of vascular aging and vascular smooth muscle cell senescence.
- COMP-Ang1-stimulated proliferation, differentiation, and migration of progenitor cells may involve the Tie-2-mediated activation of p38 MAPK and PI3K/Akt pathways
- results suggest the potential importance of COMP-Ang1 as a new therapy for impaired bone formation that is associated with diabetes and advanced age
- The presence of cleaved apoptosis inducing factor (tAIF) and the absence of activated caspases indicated that retention of D469del-COMP triggers cell death in chondrocytes by necroptosis, a caspase-independent programmed necrosis.
- Cartilage oligomeric matrix protein is a novel inhibitor of vascular smooth muscle calcification.
- Overexpression of COMP markedly suppressed PDGF-BB-elicited VSMC dedifferentiation. Alpha(7)beta(1) integrin is a potential receptor for COMP.
- Matrix-matrix interaction of cartilage oligomeric matrix protein and fibronectin.
- The X-ray structure of the COMP coiled coil domain in complex with vitamin D(3) at 1.7 A resolution is reported. COMP exerts a distinct mechanism of interaction mainly defined by the pattern of hydrophobic core residues.
- COMP has a role in development of the extracellular matrix and in a cell culture model for pseudoachondroplasia
- COMP secretion is affected by the signal peptide
- Expression of COMP in both anulus fibrosus(AF) and nucleus pulposus(NP) of intervertebral disc(IVD). COMP is component of extracellular matrix of AF and NP, with lamellar distribution in AF. May play role in normal structure of IVD.
- ultrastructural distribution of COMP & TSP-4 & collagen fibril thickness in achilles tendon related to exercise; COMP immunolabeling decreased with longer running distance & was lower in pericellular & interterritorial compartments of high training rats
- To identify residues critical for the structure, stability, oligomerization and binding to vitamin D as well as two small molecules, all-trans-retinol and curcumin, an alanine-scanning mutagenesis study of cartilage oligomeric matrix protein is performed.
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Thrombospondin family
Tissue Specificity
Expressed in differentiated and de-differentiated vascular smooth muscle cells (VSMCs) (at protein level).
Database Links
KEGG:
STRING:
UniGene: