Rat Bone morphogenetic protein 4,BMP-4 ELISA Kit
Referencia CSB-E04511r-96T
embalaje : 96T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
bone morphogenetic protein 4
Associated names
Bmp4 ELISA Kit; Bmp-4 ELISA Kit; Dvr-4Bone morphogenetic protein 4 ELISA Kit; BMP-4 ELISA Kit; Bone morphogenetic protein 2B ELISA Kit; BMP-2B ELISA Kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-105 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-100 | |||||
Recovery
| The recovery of rat BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 92 | 87-98 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Rat BMP4 ELISA Kit was designed for the quantitative measurement of Rat BMP4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
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Target Background
Function
(From Uniprot)
(From Uniprot)
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by...
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
TGF-beta family
Database Links
STRING:
UniGene: