Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with RAD21 recombinant monoclonal antibody, clone R03-5I0 (Cat # RAB01219).

Immunocytochemistry
Application

Immunocytochemistry

Immunocytochemistry staining of HeLa cells with RAD21 recombinant monoclonal antibody, clone R03-5I0 (Cat # RAB01219).

Western Blot
Application

Western Blot

Western blot analysis of Rad21 in Hela, CHO-K1, Ramos lysates using Rad21 antibody.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against human RAD21.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs,RAB,MAB,Recombinant Mab,MAB,monoclonal Ab,monoclonal antibody

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against synthetic peptide corresponding to human Rad21.

    Theoretical MW (kDa)

    Calculated MW: 72 kD

    Reactivity

    Human

    Form

    Liquid

    Preparation Method

    Mammalian cell (CHO) expression system

    Purification

    Affinity purification

    Isotype

    IgG

    Recommend Usage

    Western Blot
    Immunohistochemistry (Frozen sections)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Immunocytochemistry
    Immunofluorescence
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA).

    Storage Instruction

    Store at 4°C. For longer storage, aliquot and store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with RAD21 recombinant monoclonal antibody, clone R03-5I0 (Cat # RAB01219).

    Immunohistochemistry (Frozen sections)

    Immunofluorescence

    Immunocytochemistry

    Immunocytochemistry staining of HeLa cells with RAD21 recombinant monoclonal antibody, clone R03-5I0 (Cat # RAB01219).

    Western Blot

    Western blot analysis of Rad21 in Hela, CHO-K1, Ramos lysates using Rad21 antibody.
  • Gene Info — RAD21

    Entrez GeneID

    5885

    Protein Accession#

    O60216

    Gene Name

    RAD21

    Gene Alias

    FLJ25655, FLJ40596, HR21, HRAD21, KIAA0078, MCD1, NXP1, SCC1, hHR21

    Gene Description

    RAD21 homolog (S. pombe)

    Omim ID

    606462

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq

    Other Designations

    RAD21 homolog|nuclear matrix protein 1|protein involved in DNA double-strand break repair

  • Interactomes
  • Pathways
  • Diseases
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