Rabbit creatine kinase MB isoenzyme (CK-MB) ELISA kit
Referencia CSB-EQ027653RB-24T
embalaje : 24T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
creatine kinase MB isoenzyme (CK-MB)
Abbreviation
CK-MB
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 mU/mL-100 mU/mL
Sensitivity
0.39 mU/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-107 | |||||
Recovery
| The recovery of rabbit CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 83-96 | ||||
| EDTA plasma (n=4) | 92 | 88-98 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Rabbit CK-MB ELISA Kit was designed for the quantitative measurement of Rabbit CK-MB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 mU/mL-100 mU/mL and the sensitivity is 0.39 mU/mL.
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