Rabbit Anti-MUC5B Polyclonal Antibody

Referencia HPA008246

embalaje : 100ul

Marca : Atlas Antibodies

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HPA008246
mucin 5B, oligomeric mucus/gel-forming

Anti-MUC5B Antibody

Polyclonal Antibody against HUMAN MUC5B

0.1 mg/ml
info@quimigen.pt

Immunohistochemistry analysis in human cervix, uterine and skeletal muscle tissues using HPA008246 antibody. Corresponding MUC5B RNA-seq data are presented for the same tissues.

Product details

  • Target protein: mucin 5B, oligomeric mucus/gel-forming
  • Target gene: MUC5B
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000066108 (64%)RAT - ENSRNOG00000019846 (64%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000069924
  • Concentration: 0.1 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:500 - 1:1000
    • Retrieval method: HIER pH6

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  

Target information

  • Protein name: mucin 5B, oligomeric mucus/gel-forming
  • Gene name: MUC5B
  • Alternative gene names: MG1, MUC5, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (42)

  • Guo TJF, Singhera GK, Memar Vaghri J, Liang WY, Leung JM, Dorscheid DRPhysiol Rep , 2026 Apr; 14(7):e707442026 Apr

  • Lo Cicero S, Castelli G, Ceci A, Cerio AM, Blaconà G, Virgulti M, Allushi S, Sette G, Spadaro F, Amato F, Melotti P, Sorio C, Cimino G, Biffoni M, Lucarelli M, Eramo ACells , 2025 Dec 2; 14(23):1914. Epub 2025 Dec 22025 Dec 2

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