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> Rabbit Anti-Mouse IgG - HRP For IP/CoIP

Rabbit Anti-Mouse IgG - HRP For IP/CoIP

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Referencia CSA9107-100ul

embalaje : 100ul

Marca : Cohesion Biosciences

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Teléfono : +1 850 650 7790

  • Immunoprecipitating GAPDH in Hela whole cell lysate with mouse anti-GAPDH antibody. Western blot was performed on the immunoprecipitate using anti-GAPDH antibody, and followed by the normal Anti-Mouse IgG - HRP and Anti-Mouse IgG - HRP For IP/CoIP. Lane A and D (Input): Hela whole cell lysate; Lane B and E (-): Mouse IgG Isotype Control instead of GAPDH antibody in Hela whole cell lysate; Lane C and F (+): GAPDH antibody IP in Hela whole cell lysate.
Product Name:Anti-Mouse IgG - HRP For IP/CoIP
Cat No:CSA9107
Source:
Reactivity:M
Applications:IP, CoIP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Monoclonal Secondary Antibody to Mouse IgG HRP labeled
Immunogen：Mouse IgG
Purification：The antibody is isolated from cell culture by protein A/G immunoaffinity chromatography.
Specificity：The antibody is a conformation-specific secondary antibody that only reacts with native mouse IgG and does not bind to the denatured and reduced mouse IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured IgG light and heavy chains of the primary antibody used for IP will not be recognised.
Clonality：Monoclonal
Conjugation：HRP
Form：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.05% Proclin
Dilution：WB (1/500 - 1/2000)
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.