Rabbit Anti-FNIP2 Polyclonal Antibody

Referencia HPA042779

embalaje : 100ul

Marca : Atlas Antibodies

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HPA042779
folliculin interacting protein 2

Anti-FNIP2 Antibody

Polyclonal Antibody against HUMAN FNIP2

0.8 mg/ml
info@quimigen.pt

Immunohistochemical staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.

Product details

  • Target protein: folliculin interacting protein 2
  • Target gene: FNIP2
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000061175 (52%)RAT - ENSRNOG00000027833 (55%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000013017
  • Concentration: 0.8 mg/ml

Applications

  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:200 - 1:500
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunohistochemistry (IHC) 
  • Western Blot (WB)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. 

Target information

  • Protein name: folliculin interacting protein 2
  • Gene name: FNIP2
  • Alternative gene names: FNIPL, KIAA1450, MAPO1, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (3)

  • Glykofridis IE, Knol JC, Balk JA, Westland D, Pham TV, Piersma SR, Lougheed SM, Derakhshan S, Veen P, Rooimans MA, van Mil SE, Böttger F, Poddighe PJ, van de Beek I, Drost J, Zwartkruis FJ, de Menezes RX, Meijers-Heijboer HE, Houweling AC, Jimenez CR, Wolthuis RMeLife , 2021 Jan 18; 10:e61630. Epub 2021 Jan 182021 Jan 18

  • Nagashima K, Fukushima H, Shimizu K, Yamada A, Hidaka M, Hasumi H, Ikebe T, Fukumoto S, Okabe K, Inuzuka HOncotarget , 2016 Dec 25; 8(6):9947-9960. Epub 2016 Dec 252016 Dec 25