Protein G Rabbit Polyclonal Antibody

Referencia AP09166FC-N

embalaje : 20mg

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Protein G Rabbit Polyclonal Antibody

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SKU
AP09166FC-N
Protein G rabbit polyclonal antibody, FITC
 
Specifications
Product Data
Application FC, IF
Recommended Dilution Immunofluorescence: 1/500 - 1/2500.
Flow Cytometry: 1/2000 - 1/10000.
Reactivity Streptococcus
Antibody Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Protein G Streptococcus species
Specificity This antibody reacts to Protein G.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% Thimerosal as preservative
Label: FITC
State: Lyophilized IgG fraction
Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
Absorption emission: 495 nm / 528 nm
Reconstitution Method Restore with 2.0 ml of deionized water (or equivalent).
Concentration lot specific
Purification Ion exchange chromatography
Conjugation FITC
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Background Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
Note Physical State: Lyophilized
Reference Data

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