Porcine Cytokine Array Q50 (Glass Slide)

Referencia QAP-CAA-50-2

embalaje : 22SampleKit

Marca : RayBiotech

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Porcine Cytokine Array Q50

Quantibody® Porcine Cytokine Antibody Array 50 Kit. A combination of 5 non-overlapping arrays to quantitatively measure 50 Porcine cytokines. Suitable for all liquid sample types.
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Product Description

Specifications

Size8 Sample Kit, 22 Sample Kit, 50 Sample Kit
SpeciesPorcine (pig)
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeQuantitative
Number of Targets Detected50
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Shipping TypeBlue ice
Storage-20°C

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Angiopoietin-1
  • Gene Symbol: ANGPT1
  • Accession #: F1S1F8
  • ELISA Kit: RayBio Porcine Angiopoietin-1 ELISA
CCL3L1
  • Gene Symbol: ANGPT1
  • Accession #: F1S1F8
  • ELISA Kit: RayBio Porcine Angiopoietin-1 ELISA
Decorin
  • Accession #: A1YM34
  • ELISA Kit: RayBio Custom Porcine CCL3L1 ELISA
Eotaxin-1
(CCL11)
  • Gene Symbol: DCN
  • Gene ID: 396957
  • Accession #: Q9XSD9
  • ELISA Kit: RayBio Porcine Decorin ELISA
Erythropoietin
  • Accession #:
  • ELISA Kit: RayBio Custom Porcine CCL11 ELISA
FGF-21
  • Gene Symbol: EPO
  • Gene ID: 397249
  • Accession #: P49157
  • ELISA Kit: RayBio
Galectin-9
  • Gene Symbol: FGF21
  • Gene ID: 100302504
  • Accession #: C6JWG4
  • ELISA Kit: RayBio
GASP-1
(WFIKKNRP)
GM-CSF
  • Accession #:
  • ELISA Kit: RayBio Custom Porcine GASP-1 ELISA
IFN alpha
  • Gene Symbol: CSF2
  • Gene ID: 397208
  • Accession #: Q29118
  • ELISA Kit: RayBio Porcine GM-CSF ELISA
IFN-beta
  • Gene ID:
  • Accession #: P49879
  • ELISA Kit: RayBio Porcine IFN-alpha ELISA
IFN-gamma
  • Gene Symbol: interferon-beta-1 IFN-beta IFNB IFNB1 interferon-beta/ poIFN-beta
  • Accession #: Q29412
  • ELISA Kit: RayBio Porcine IFN-beta ELISA
IGF-2
  • Gene Symbol: IFNG
  • Gene ID: 396991
  • Accession #: P17803
  • ELISA Kit: RayBio Porcine IFN-gamma ELISA
IGFBP-5
  • Gene Symbol: IGF2
  • Gene ID: 396916
  • Accession #: P23695
  • ELISA Kit: RayBio
IL-1 alpha
(IL-1 F1)
  • Gene Symbol: IGFBP5
  • Gene ID: 397182
  • Accession #: Q28985
  • ELISA Kit: RayBio Custom Porcine IGFBP-5 ELISA
IL-1 beta
(IL-1 F2)
  • Gene Symbol: IL1A
  • Gene ID: 397094
  • Accession #: P18430
  • ELISA Kit: RayBio Porcine IL-1 alpha/IL-1 F1 ELISA
IL-1 Ra
(IL-1 F3)
  • Gene Symbol: IL1B
  • Gene ID: 397122
  • Accession #: P26889
  • ELISA Kit: RayBio Porcine IL-1 beta ELISA
IL-10
  • Gene Symbol: IL1RN IRAP1
  • Gene ID: 397499
  • Accession #: Q29056
  • ELISA Kit: RayBio Custom Porcine IL-1 Ra ELISA
IL-12 p70
  • Gene Symbol: IL12A
  • Gene ID: 397053
  • Accession #: Q29053
  • ELISA Kit: RayBio Porcine IL-12 ELISA
IL-13
  • Gene Symbol: IL13
  • Gene ID: 396721
  • Accession #: Q95J68
  • ELISA Kit: RayBio Porcine IL-13 ELISA
IL-15
  • Gene Symbol: IL15
  • Gene ID: 397683
  • Accession #: Q95253
  • ELISA Kit: RayBio Custom Porcine IL-15 ELISA
IL-17A
  • Gene Symbol: IL17A IL17
  • Gene ID: 449530
  • Accession #: Q60I29
  • ELISA Kit: RayBio Porcine IL-17 ELISA
IL-17F
  • Gene Symbol: IL17F
  • Accession #: F1RKV0
  • ELISA Kit: RayBio Porcine IL-17F ELISA
IL-18
  • Gene Symbol: IL18 IGIF
  • Gene ID: 281249
  • Accession #: Q9TU73
  • ELISA Kit: RayBio Porcine IL-18 ELISA
IL-21
  • Gene Symbol: IL21
  • Gene ID: 403123
  • Accession #: Q76LU6
  • ELISA Kit: RayBio
IL-22
  • Gene Symbol: IL22
  • Gene ID: 595104
  • Accession #: K7GM74
  • ELISA Kit: RayBio Porcine IL-22 ELISA
IL-28B
  • Gene Symbol: IL28B
  • Gene ID: 100310828
  • Accession #: D0FHA1
  • ELISA Kit: RayBio
IL-4
  • Gene Symbol: IL4
  • Gene ID: 397225
  • Accession #: Q04745
  • ELISA Kit: RayBio Porcine IL-4 ELISA
IL-6
  • Gene Symbol: IL6
  • Gene ID: 100628202; 399500
  • Accession #: P26893
  • ELISA Kit: RayBio Porcine IL-6 ELISA
IL-8
(CXCL8)
  • Gene Symbol: CXCL8 IL8
  • Gene ID: 100620730; 396880
  • Accession #: P26894
  • ELISA Kit: RayBio Porcine IL-8 ELISA
Insulin
  • Gene Symbol: INS
  • Gene ID: 397415
  • Accession #: P01315
  • ELISA Kit: RayBio Porcine Insulin ELISA
IP-10
(CXCL10)
  • Accession #: Q2EN90
  • ELISA Kit: RayBio Porcine IP-10 ELISA
MCP-1
(CCL2)
  • Gene Symbol: CCL2 MCP1 SCYA2
  • Gene ID: 100135494
  • Accession #: Q08782
  • ELISA Kit: RayBio Porcine CCL2/MCP-1 ELISA
MIF
  • Gene Symbol: MIF
  • Gene ID: 397412
  • Accession #: P80928
  • ELISA Kit: RayBio Porcine MIF ELISA
MIG
(CXCL9)
  • Gene Symbol: CXCL9
  • Gene ID: 100135681
  • Accession #: B0FYK2
  • ELISA Kit: RayBio Porcine CXCL9 ELISA
MIP-1 beta
(CCL4)
  • Gene Symbol: CCL4 MIP1B SCY4A
  • Gene ID: 396668
  • Accession #: Q711P4
  • ELISA Kit: RayBio Porcine CCL-4/MIP-1 beta ELISA
NCAM-1
(CD56)
  • Gene Symbol: NCAM1
  • Accession #: F1SM72
  • ELISA Kit: RayBio Custom Porcine NCAM-1 ELISA
Osteoprotegerin
(TNFRSF11B)
  • Gene Symbol: TNFRSF11B
  • Gene ID: 100738370
  • Accession #: F1S278
  • ELISA Kit: RayBio Porcine OPG ELISA
PDGF-BB
  • Gene Symbol: PDGFB
  • Gene ID:
  • Accession #: P20034
  • ELISA Kit: RayBio Porcine PDGF-BB ELISA
PECAM-1
(CD31)
  • Gene Symbol: PECAM1
  • Accession #: K7GPI3
  • ELISA Kit: RayBio Custom Porcine PECAM-1 ELISA
PLGF-2
RANTES
(CCL5)
  • Gene Symbol: CCL5 SCYA5
  • Gene ID:
  • Accession #: Q29288
  • ELISA Kit: RayBio Porcine RANTES ELISA
SCF
  • Gene Symbol: KITLG MGF
  • Gene ID: 100626805 397509
  • Accession #: Q29030
  • ELISA Kit: RayBio
TGF alpha
  • Accession #:
  • ELISA Kit: RayBio Porcine TGF alpha ELISA
TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 397078
  • Accession #: P07200
  • ELISA Kit: RayBio
TIMP-1
  • Gene Symbol: TIMP1
  • Gene ID: 396862
  • Accession #: P35624
  • ELISA Kit: RayBio Porcine TIMP-1 ELISA
TIMP-2
  • Gene Symbol: TIMP-2 TIMP2
  • Gene ID: 396988;
  • Accession #: C0JPM4
  • ELISA Kit: RayBio Porcine TIMP-2 ELISA
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 397086
  • Accession #: P23563
  • ELISA Kit: RayBio Porcine TNF-alpha ELISA
TWEAK R
(TNFRSF12)
  • Gene Symbol: TNFRSF12A
  • Gene ID: 100217390;
  • Accession #: B7SLY2
  • ELISA Kit: RayBio Custom Porcine TWEAK R ELISA

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Porcine Cytokine Array Q50 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAP-CYT-1.gal)
GAL (GAL-QAP-CYT-2.gal)
GAL (GAL-QAP-CYT-3.gal)
GAL (GAL-QAP-CYT-4.gal)
GAL (GAL-QAP-CYT-5.gal)
Excel (QAP-CYT-1_Analysis_Tool.xls)
Excel (QAP-CYT-2_Analysis_Tool.xls)
Excel (QAP-CYT-3_Analysis_Tool.xls)
Excel (QAP-CYT-4_Analysis_Tool.xls)
Excel (QAP-CYT-5_Analysis_Tool.xls)

citations

  • Optimized Centrifugation and Activation Protocol for the Preparation of Plasma Rich in Growth Factors in Pigs

    Species:
    Porcine
    Sample Type:
    Cell Culture Supernatant (supernatants of PRGF activated with 13.3 mM CaCl2)
    Year:
    2026
    Journal Name:
    Biomedicines
    PubMed ID:
    41898286

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Referencia
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Precio Sin IVA
22003
 200each