Polyclonal Rabbit Anti-Mouse IgM Secondary Antibody (Biotin, IHC, WB)
Referencia LS-C60804-1.5
embalaje : 1.5mg
Marca : LSBio
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Teléfono : +1 850 650 7790
Overview
Antibody:
Mouse IgM Rabbit anti-Mouse Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Biotin, Unmodified
Specifications
Description
Mouse IgM antibody LS-C60804 is a biotin-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Target
Mouse IgM
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Mouse IgM whole molecule.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgM and Mouse Serum. No reactivity was observed against other mouse heavy or light chain proteins.
Applications
- IHC (1:1000 - 1:5000)
- Western blot (1:2000 - 1:10000)
- ELISA (1:20000 - 1:100000)
Usage
Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
tech@quimigen.pt