Western Blot (Tissue lysate)
Western blot analysis of PLIN1 in human liver samples with PLIN1 polyclonal antibody (Cat # PAB12071).
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PLIN1.
Immunogen
A synthetic peptide corresponding to amino acids 450-522 of human PLIN1.
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Pig, Rat, Sheep
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Tissue lysate)
Western blot analysis of PLIN1 in human liver samples with PLIN1 polyclonal antibody (Cat # PAB12071). - Gene Info — PLIN
Entrez GeneID
5346Protein Accession#
O60240Gene Name
PLIN
Gene Alias
PERI
Gene Description
perilipin
Omim ID
170290Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq
Other Designations
lipid droplet-associated protein
- Interactomes
- Pathways
- Diseases
- Publication Reference
- Expression of perilipin in human promyelocytic cells in response to Anaplasma phagocytophilum infection results in modified lipid metabolism.
Manzano-Roman R, Almazan C, Naranjo V, Blouin EF, Kocan KM, de la Fuente J.
Journal of Medical Microbiology 2008 Feb; 57(Pt 2):159.
Application：IF, Human, HL-60 cells.
- Evolutionarily conserved gene family important for fat storage.
Kadereit B, Kumar P, Wang WJ, Miranda D, Snapp EL, Severina N, Torregroza I, Evans T, Silver DL.
PNAS 2008 Jan; 105(1):94.
Application：WB, Mouse, 3T3-L1 cells, Lipid droplets and membranes from mouse brown adipose tissues.
- Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein.
Brasaemle DL, Barber T, Wolins NE, Serrero G, Blanchette-Mackie EJ, Londos C.
Journal of Lipid Research 1997 Nov; 38(11):2249.
Application：IF, WB-Ce, Mouse, 3T3-L1, MA-10 Leydig cells.
- Expression of perilipin in human promyelocytic cells in response to Anaplasma phagocytophilum infection results in modified lipid metabolism.