Lentivirus vector based on the human immunodeficiency virus-1 (HIV-1) has become a promising vector for gene transfer studies. Lentiviral vectors packaged as lentiviral particles is one of the most efficient tools to deliver exogenous genes into virtually any types of mammalian cells both in vitro and in vivo. The advantageous feature of lentivirus vector is the ability of gene transfer and integration into dividing and non-dividing cells, with low immune response and toxicity in vivo. These viruses also integrate stably into the host genome, enabling long-term transgene expression. Our 3rd generation lentiviral systems have been designed for increased researcher safety. pLenti-EF1-Luciferase-PGK-GFP-T2A-Puro Lentiviral Reporter Plasmid contains Luciferase reporter driven by EF1α promoter, GFP and Puromycin resistance gene are driven by PGK promoter respectively. This dual reporter vector serves a positive control. The cells transduced by this vector should display green fluorescence and luciferase.