phospho-PTPN6 (Tyr536) Polyclonal Antibody
Referencia bs-5577R
embalaje : 100uL
Marca : Bioss
Contact local distributor :
Teléfono : +1 850 650 7790
Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with PTPN6(Tyr536) Polyclonal Antibody (bs-5577R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5577R |
| Product Name | phospho-PTPN6 (Tyr536) Polyclonal Antibody |
| Applications | WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC) |
| Reactivity | Human |
| Predicted Reactivity | Dog, Horse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PTPN6 around the phosphorylation site of Tyr536 [SE(p-Y)GN] |
| Modification Site | Tyr536 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 5777 |
| Swiss Prot | P29350 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | SHP-1Tyr536; PTPN6phospho Y536; SHP1phospho Y536; 70 kda SHP1L protein; HCP; HCPH; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP1C; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase non receptor type 6; Protein tyrosine phosphatase SHP1; PTP1C; PTPN6; SH PTP 1; SHP1; SHP1L; PTN6_HUMAN. |
| Background | SHP1 is a member of the non receptor protein tyrosine phosphatase subfamily. The PTP family comprises of at least 37 proteins, characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. PTP1B is cytosolic. The PTPs have high substrate specificity for phosphotyrosyl proteins, at the primary sequence level sharing little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. SHP1 is implicated in the control of tyrosine kinase signalling pathways in cellular proliferation, with a potential role in cancer. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
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Precio Sin IVA
PG067
500mL/bottle