Use code ELISA25-262 for 25% off all ELISAs through November.
25% off all ELISA kits
Phospho-AKT (S473) ELISA Kit
Phospho-AKT (S473) ELISA Kit
The RayBiotech Phospho-AKT (S473) ELISA Kit measures phosphorylated AKT (Ser473) and total (pan) AKT in cell and tissue lysates.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human, Mouse, Rat |
| Specificity | The antibody pair provided in this kit recognizes human, mouse and rat Phospho-Akt (pSer473). |
| Compatible Sample Types | Tissue Lysates, Cell Lysates |
| Accession Number | P31749 |
| Gene Id | 391 |
| Gene Symbols | AKT1, PKB, RAC |
| Protein Name / Synonyms | RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha) |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Apoptosis, Post-Translational Modifications, Inflammation, Phosphorylation, mTOR Signaling, PI3K-AKT Signaling, Insulin Signaling, Autophagy, Akt Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Related Products
Human/Mouse/Rat AKT (S473) Cell-Based ELISA Kit
As low as $397.00
Anti-Phospho-AKT1 (S473) Antibody
$351.00
Human/Mouse AKT Pathway Phosphorylation Array C1
As low as $342.00
Product Features
- Simultaneously measure phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody (*not included in phospho-only version)
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
Other Materials Required
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
- Prepare all reagents and samples as instructed in the manual.
- Add 100 µl of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 µl of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
documents
citations
Effect of curcumin-loaded polydopamine nanoparticles on the expression of AKT, HDAC6, and HSF1 in Trembler-J Schwann cell cultures
- Species:
- Mouse
- Sample Type:
- Cell Lysate (wt, TrJ schwann cell lysates); Tissue Lysate / Homogenate
- Year:
- 2026
- Journal Name:
- Research Square
Novel insights into gut health: Cilostazol strengthens gut integrity by adjusting TLR-2/NF-κB/IL-23 and CD44/AKT/GSK-3β/cyclin-D1 trajectories in methotrexate-induced mucositis model
- Year:
- 2024
- Journal Name:
- European Journal of Pharmacology
- PubMed ID:
- 38795758
Local application of engineered insulin-like growth factor I mRNA demonstrates regenerative therapeutic potential in vivo
- Species:
- Mouse, Rat
- Sample Type:
- muscle tissue
- Year:
- 2023
- Journal Name:
- Molecular Therapy-Nucleic Acids
Renoprotective Effect of Pitavastatin against TAA-Induced Renal Injury: Involvement of the miR-93/PTEN/AKT/mTOR Pathway
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (renal tissue homogenate)
- Year:
- 2024
- Journal Name:
- Advances in pharmacological and pharmaceutical sciences
- PubMed ID:
- 38293706
Dapagliflozin/Hesperidin Combination Mitigates Lipopolysaccharide-Induced Alzheimer’s Disease in Rats
- Species:
- Rat
- Sample Type:
- hippocampal
- Year:
- 2023
- Journal Name:
- Pharmaceuticals
The Role of Beta-Hydroxybutyrate in Mitigating the Inflammatory and Metabolic Consequences of Uric Acid
- Species:
- Mouse
- Sample Type:
- Cell Lysate (cell lysates C2C12 murine muscle cells); Tissue Lysate / Homogenate
- Year:
- 2024
- Journal Name:
- Metabolites
- PubMed ID:
- 39728460
Empagliflozin enhances neuroplasticity in rotenone-induced parkinsonism: Role of BDNF, CREB and Npas4
- Species:
- Mouse
- Sample Type:
- brain
- Year:
- 2022
- Journal Name:
- LIFE SCIENCES
Glimepiride mitigates tauopathy and neuroinflammation in P301S transgenic mice: role of AKT/GSK3β signaling
- Species:
- Mouse
- Sample Type:
- brain
- Year:
- 2022
- Journal Name:
- INFLAMMOPHARMACOLOGY
Mitoquinone Alleviates Donation after Cardiac Death Kidney Injury during Hypothermic Machine Perfusion in Rat Model
- Species:
- Rat
- Sample Type:
- kidney tissue
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
Icosapent ethyl alleviates acetic acid-induced ulcerative colitis via modulation of SIRT1 signaling pathway in rats
- Species:
- Rat
- Sample Type:
- colon
- Year:
- 2022
- Journal Name:
- International Immunopharmacology
