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Dispatch Time: About 3 working days
- Product Name
- PerCP-Cy5.5 Anti-Human CD25 Antibody(BC96)
- Catalogue No.
- PCP55-30035
- Form
- liquid
- Conjugation
- PerCP-Cy5.5
- Conjugation Information
- PerCP-Cy5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).
- Clonality
- Monoclonal
- Isotype
- IgG1, κ
- Clone ID
- BC96
- Storage
- PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Alternative Names
- IL-2 receptor subunit alpha|IL-2-RA|IL-2R subunit alpha|IL2-RA|IL2RA|Interleukin-2 receptor subunit alpha|TAC antigen|p55 antibody
- UniProt ID
- P01589
Application
- Tested Applications
- FC
- Recommended dilution
- Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Validated Images
Human peripheral blood lymphocytes are stained with FITC Anti-Human CD4 Antibody and PerCP-Cy5.5 Anti-Human CD25 Antibody (Left). Lymphocytes are stained with FITC Anti-Human CD4 Antibody and PerCP/Cy5.5 Mouse IgG1, κ Isotype Control (Right). - Background
- CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.