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- Product Name
- PE Anti-Mouse CD8b.2 Antibody(53-5.8)
- Catalogue No.
- PE-30196
- Form
- liquid
- Conjugation
- PE
- Conjugation Information
- PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
- Clonality
- Monoclonal
- Isotype
- IgG1, κ
- Clone ID
- 53-5.8
- Storage
- PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Alternative Names
- Lyt-3.2|Ly-3.2 antibody
- UniProt ID
- P10300
Application
- Tested Applications
- FC
- Recommended dilution
- Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Validated Images
Staining of C57BL/6 murine splenocytes cells with APC Anti-Mouse CD8a Antibody and PE Anti-Mouse CD8b.2 Antibody(53-5.8) (left) or PE Rat IgG1, κ Isotype Control (right). Total viable cells were used for analysis. - Background
- CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.