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- Product Name
- PE Anti-Mouse CD366/Tim-3 Antibody(RMT3-23)
- Catalogue No.
- PE-30273
- Form
- liquid
- Conjugation
- PE
- Conjugation Information
- PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
- Clonality
- Monoclonal
- Isotype
- IgG2a, κ
- Clone ID
- RMT3-23
- Storage
- PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Alternative Names
- TIMD3|HAVcr-2|TIM3|TIMD-3 antibody
- UniProt ID
- Q8VIM0
Application
- Tested Applications
- FC
- Recommended dilution
- Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Validated Images
C57BL/6 murine splenocytes are stained with APC Anti-Mouse CD4 Antibody and PE Anti-Mouse CD366/Tim-3 Antibody (Left). Splenocytes are stained with APC Anti-Mouse CD4 Antibody and PE Rat IgG2a, κ Isotype Control (Right). - Background
- CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.