NFKB1 ELISA Kit (Chicken)
Referencia OKCD02756
embalaje : 96Wells
Marca : Aviva Systems Biology
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Teléfono : +1 850 650 7790
NFKB1 ELISA Kit (Chicken) (OKCD02756)
| Datasheets/Manuals | Printable datasheet for NFKB1 ELISA Kit (Chicken) (OKCD02756) |
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| Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Kappa B (NFkB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor Kappa B (NFkB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor Kappa B (NFkB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nuclear Factor Kappa B (NFkB) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Factor Kappa B (NFkB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Factor Kappa B (NFkB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.057 ng/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Nuclear Factor Kappa B (NFkB). No significant cross-reactivity or interference between Nuclear Factor Kappa B (NFkB) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
| Protocol Information |
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| Gene Symbol | NFKB1 |
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| Gene Full Name | nuclear factor kappa B subunit 1 |
| Alias Symbols | NF-kB p50 subunit;NF-KB1;nuclear factor NF-kappa-B p105 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1;nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105). |
| NCBI Gene Id | 396033 |
| Protein Name | Nuclear factor NF-kappa-B p105 subunit |
| Description of Target | P105 is the precursor of the p50 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. The precursor protein itself does not bind to DNA. |
| Uniprot ID | Q04861 |
| Protein Accession # | NP_990465.1 |
| Nucleotide Accession # | NM_205134.1 |
| Protein Size (# AA) | 983 |
