General Information
| Short Description | This SHH antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rat/Rat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen | Capture immunogen: Recombinant Mouse SHH protein.Detection Immunogen: Recombinant Mouse SHH protein. |
Additional Info
| Post Translational Modifications | Sonic hedgehog protein: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (ShhN). Cholesterylation is required for the sonic hedgehog protein N-product targeting to lipid rafts and multimerization. ShhN is the active species in both local and long-range signaling, whereas the C-product (ShhC) is degraded in the endoplasmic reticulum. Sonic hedgehog protein N-product: N-palmitoylation by HHAT of ShhN is required for sonic hedgehog protein N-product multimerization and full activity. It is a prerequisite for the membrane-proximal positioning and the subsequent shedding of this N-terminal peptide. Sonic hedgehog protein N-product: The lipidated N- and C-terminal peptides of ShhNp can be cleaved (shedding). The N-terminal palmitoylated peptide is cleaved at the Cardin-Weintraub (CW) motif site. The cleavage reduced the interactions with heparan sulfate. The cleavage is enhanced by SCUBE2. |
| Function | Sonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum. Sonic hedgehog protein N-product: The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites. Involved in the patterning of the anterior-posterior axis of the developing limb bud. Essential for axon guidance. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO. |
| Protein Name | Sonic Hedgehog ProteinShhHhg-1Shh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing DomainsShhnc Cleaved Into - Sonic Hedgehog Protein N-ProductShhnShh N-Terminal Processed Signaling DomainsShhnpSonic Hedgehog Protein 19 Kda Product |
| Database Links | |
| Cellular Localisation | Sonic Hedgehog Protein: Endoplasmic Reticulum MembraneGolgi Apparatus MembraneCo-Localizes With Hhat In The Er And Golgi MembraneSonic Hedgehog Protein N-Product: Cell MembraneLipid-AnchorThe Dual-Lipidated Sonic Hedgehog Protein N-Product (Shhnp) Is Firmly Tethered To The Cell Membrane Where It Forms MultimersFurther Solubilization And Release From The Cell Surface Seem To Be Achieved Through Different MechanismsIncluding The Interaction With Disp1 And Scube2Movement By Lipoprotein ParticlesTransport By Cellular Extensions Called Cytonemes Or By The Proteolytic Removal Of Both Terminal Lipidated Peptides |
| Alternative Antibody Pair Names | Sonic Hedgehog Protein antibody pairShh antibody pairHhg-1 antibody pairShh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing Domains antibody pairShhnc Cleaved Into - Sonic Hedgehog Protein N-Product antibody pairShhn antibody pairShh N-Terminal Processed Signaling Domains antibody pairShhnp antibody pairSonic Hedgehog Protein 19 Kda Product antibody pairShh antibody pairHhg1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse SHH (Sonic Hedgehog Protein) Antibody Pair (STJAP00308)
This antibody pair is designed to detect mouse Sonic Hedgehog Protein (SHH), corresponding to UniProt entry Q62226. Both the capture and detection antibodies were raised against recombinant mouse SHH protein, and reactivity is confirmed for mouse samples only.
The capture antibody is unconjugated, while the detection antibody is biotinylated. Both are rat monoclonal antibodies supplied at 0.5 mg/mL, purified by Protein A or G.
This pair has been validated for use in ELISA. No other assay formats are listed for this product.
Both antibodies are formulated in PBS containing 0.04% Proclin 300 and 50% glycerol at pH 7.4. The formulations for the capture and detection components are identical.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.