Mouse neutrophil elastase, NE ELISA Kit
Referencia MBS701009-48
embalaje : 48-Strip-Wells
Marca : MyBiosource
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Teléfono : +1 850 650 7790
Elastase 2, Neutrophil (ELA2) ELISA Kit for Mouse
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS701009Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NE present is bound by the immobilized… More details
Product Overview
Online Datasheet in PDFProduct Name
elastase 2, neutrophil (ELA2), ELISA Kit
Full Product Name
Mouse neutrophil elastase, NE ELISA Kit
Product Gene Name
ELA2 elisa kit
Product Synonym Names
Mouse neutrophil elastase; NE ELISA Kit; ELA2; GE; HLE; HNE; NE; PMN-E; SCN1; bone marrow serine protease; elastase 2; neutrophil; granulocyte-derived elastase; leukocyte elastase; medullasin; neutrophil elastase; polymorphonuclear elastase; elastase 2; neutrophil
Species Reactivity
Mouse
Assay Type
Quantitative Sandwich
Samples
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.312 ng/ml -20 ng/ml
Sensitivity
<0.078 ng/ml.
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse NE. No significant cross-reactivity or interference between mouse NE and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Molecular Weight
28,518 Da
OMIM
202700
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of ELA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ELA2 purchase
MBS701009 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the elastase 2, neutrophil (ELA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ELA2. The ELISA analytical biochemical technique of the MBS701009 kit is based on ELA2 antibody-ELA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ELA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ELA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for ELA2. It may not necessarily be applicable to this product.
NCBI Official Full Name
neutrophil elastase preproprotein
NCBI Official Synonym Full Names
elastase, neutrophil expressed
NCBI Official Symbol
ELANE
NCBI Official Synonym Symbols
GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
UniProt Protein Name
Neutrophil elastase
UniProt Synonym Protein Names
Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase
UniProt Gene Name
ELANE
UniProt Synonym Gene Names
ELA2; HLE
UniProt Entry Name
ELNE_HUMAN
NCBI Protein Information
neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase
NCBI Summary for ELA2
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]
Show more UniProt Comments for ELA2
Function: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.1 PublicationManual assertion based on experiment in:Ref.15
Catalytic activity: Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa > Ala-|-Xaa.
Show more Catalytic activity: Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa > Ala-|-Xaa.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with ELA2 elisa kit
| Reference Product | PubMed Publications |
|---|---|
| MPO elisa kit | >2 publications with ELA2 and MPO |
Pathways associated with ELA2 elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Activation Of Matrix Metalloproteinases Pathway antibodies | Activation Of Matrix Metalloproteinases Pathway Diagram |
| C-MYB Transcription Factor Network Pathway antibodies | C-MYB Transcription Factor Network Pathway Diagram |
| Collagen Degradation Pathway antibodies | Collagen Degradation Pathway Diagram |
| Degradation Of The Extracellular Matrix Pathway antibodies | Degradation Of The Extracellular Matrix Pathway Diagram |
| Extracellular Matrix Organization Pathway antibodies | Extracellular Matrix Organization Pathway Diagram |
| Systemic Lupus Erythematosus Pathway antibodies | Systemic Lupus Erythematosus Pathway Diagram |
| Systemic Lupus Erythematosus Pathway antibodies | Systemic Lupus Erythematosus Pathway Diagram |
| Transcriptional Misregulation In Cancer Pathway antibodies | Transcriptional Misregulation In Cancer Pathway Diagram |
| Transcriptional Misregulation In Cancer Pathway antibodies | Transcriptional Misregulation In Cancer Pathway Diagram |
| Amb2 Integrin Signaling Pathway antibodies | Amb2 Integrin Signaling Pathway Diagram |
Diseases associated with ELA2 elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Neutropenia Antibodies | >39 publications with ELA2 and Neutropenia |
| Cyclic neutropenia Antibodies | >8 publications with ELA2 and Cyclic neutropenia |
| Neutropenia, Severe Congenital, Autosomal Dominant 1 Antibodies | >5 publications with ELA2 and Neutropenia, Severe Congenital, Autosomal Dominant 1 |
| Lung Diseases Antibodies | >4 publications with ELA2 and Lung Diseases |
| Disease Models, Animal Antibodies | >2 publications with ELA2 and Disease Models, Animal |
| Weight Loss Antibodies | >2 publications with ELA2 and Weight Loss |
| Inflammation Antibodies | >2 publications with ELA2 and Inflammation |
| Shock Antibodies | >1 publications with ELA2 and Shock |
| Liver Diseases Antibodies | >1 publications with ELA2 and Liver Diseases |
| Pain Antibodies | >1 publications with ELA2 and Pain |
Organs/Tissues associated with ELA2 elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >12 publications with ELA2 and Blood |
| Bone Marrow Antibodies | >4 publications with ELA2 and Bone Marrow |
| Lung Antibodies | >3 publications with ELA2 and Lung |
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