Mouse IgM, Human ads Antibody : Biotin
Referencia OASB01486
embalaje : 0.5mg
Marca : Aviva Systems Biology
Contact local distributor :
Teléfono : +1 850 650 7790
Goat Anti-Mouse IgM Antibody - Biotin Conjugated (OASB01486)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Mouse IgM Antibody - Biotin Conjugated (OASB01486) |
|---|
| Tested Species Reactivity | Mouse |
|---|---|
| Predicted Species Reactivity | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | Biotin |
| Application | FC, IHC-F, IHC-P, ICC |
| Additional Information | Description: Pepsin digest of Goat Anti-Mouse IgM, Human ads - Pooled antisera from goats hyperimmunized with mouse IgM; purified by affinity chromatography on mouse IgM covalently linked to agarose; reacts with the heavy chain of mouse IgM; cross-adsorbed against mouse IgG and IgA / human immunoglobulins and pooled sera for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Mouse IgM |
| Purification | Gel filtration chromatography of pepsin digested antibody. |
| Concentration | 0.5 mg/mL |
| Specificity | IgM |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flowcytometry for conformance with characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence: ELISA: FITC conjugate BIOT conjugate R-PE conjugate BIOT conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.1 ug/106 cells 1:5,000-1:10,000 |
| Application Info | Direct immunofluorescent staining of IgM+ mouse B lymphocytes, Indirect immunofluorescent staining in conjunction with mouse IgM monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - With the exception of the low endotoxin, azide- free product, the vials should be stored at 2- 8 C Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquotand freeze the low endotoxin, azide- free product at - 20 C immediately upon receipt. Each reagent isstable for the period shown on the bottle label if stored as directed. |
| Cross Absorption | Mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified humanparaproteins. |
