Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western blotting as well as other anti IgG1 antibody based assays. Recommended Dilutions: ELISA: 1/300,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/500-1/2,500.
Reactivity
Mouse
Antibody Host
Rabbit
Clonality
Polyclonal
Immunogen
Mouse IgG1 gamma 1 heavy chain
Specificity
This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA against other Mouse or Human heavy chain isotypes. Cross reactivity with Mouse IgG2b and IgG3 is negligible (< 0.5%). Cross reactivity with Mouse IgG2a and IgM is minimal.
Restore with 1.0 ml of deionized water (or equivalent).
Concentration
lot specific
Purification
Immunoaffinity Chromatography
Conjugation
HRP
Storage
Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
