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> Mouse HGF ELISA Kit

Mouse HGF ELISA Kit

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Referencia ELM-HGF-1

embalaje : 1PlateKit

Marca : RayBiotech

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Teléfono : +1 850 650 7790

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Mouse HGF ELISA Kit

Mouse HGF ELISA Kit

RayBio® Mouse HGF ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesMouse
Sensitivity
400 pg/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
400 pg/ml - 60 ng/ml
Specificity
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Recommended Dilution (Serum/Plasma)
2 - 10 fold
Accession Number
Q08048
Gene Id
15234
Gene Symbols
HGF
Protein Name / Synonyms
Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain Hepatocyte growth factor beta chain]
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaInflammation
Shipping TypeBlue ice
Storage≤-20°C
Sample typeCell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Mouse HGF ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified HGF, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

citations

  • LNP-RNA-engineered adipose stem cells for accelerated diabetic wound healing

    Species:
    Mouse
    Sample Type:
    Cell Culture Supernatant (conditioned medium of ASCs)
    Year:
    2024
    Journal Name:
    Nature Communications
    PubMed ID:
    38272900

  • Enhancement of adult liver regeneration in mice through the hepsin-mediated epidermal growth factor receptor signaling pathway

    Species:
    Mouse
    Sample Type:
    Serum
    Year:
    2024
    Journal Name:
    Communications Biology
    PubMed ID:
    39702454

  • Anti-inflammatory effect of HGF responses to oral traumatic ulcers using an HGF-Tg mouse model

    Species:
    Mouse
    Sample Type:
    Serum; Tissue Lysate / Homogenate (mouse oral mucosa tissue protein extract)
    Year:
    2022
    Journal Name:
    Experimental animals
    PubMed ID:
    34819402

  • Mesenchymal stem cells induce dendritic cell immune tolerance via paracrine hepatocyte growth factor to alleviate acute lung injury

    Species:
    Mouse
    Sample Type:
    Cell Culture Supernatant (MSC culture supernatants)
    Year:
    2019
    Journal Name:
    Stem Cell Research & Therapy
    PubMed ID:
    31801626

  • Dendritic cell-mediated survival signals inEm-Myc B-cell lymphoma depend on the transcription factor C/EBPb

    Species:
    Human
    Sample Type:
    Conditioned Media
    Year:
    2014
    Journal Name:
    Nature Communications

  • Efficacy of epicardially delivered adipose stroma cell sheets in dilated cardiomyopathy.

    Species:
    Mouse
    Sample Type:
    Tissue Lysate (dilated cardiomyopathy model)
    Year:
    2013
    Journal Name:
    Cardiovascular Research
    PubMed ID:
    23771945

  • Effect of sorafenib on murine liver regeneration.

    Species:
    Mouse
    Sample Type:
    Tissue lysate
    Year:
    2011
    PubMed ID:
    21274878

  • Upregulation of PDGF Mediates Robust Liver Regeneration after Nanosecond Pulsed Electric Field Ablation by Promoting the HGF/c-Met Pathway

    Species:
    Mouse
    Sample Type:
    Tissue Lysate / Homogenate (liver tissues)
    Year:
    2020
    Journal Name:
    Biomed Research International
    PubMed ID:
    32258116

  • Low Oxygen Tension Enhances Proliferation and Maintains Stemness of Adipose Tissue-Derived Stromal Cells.

    Species:
    Mouse
    Sample Type:
    Conditioned Media (ASCs)
    Year:
    2013
    Journal Name:
    BioResearch open access
    PubMed ID:
    23741631

  • Skeletal myofiber VEGF is necessary for myogenic and contractile adaptations to functional overload of the plantaris in adult mice

    Species:
    Mouse
    Sample Type:
    Tissue Lysate (Skeletal muscle lysate from plantaris muscle)
    Year:
    2016
    Journal Name:
    Journal of Applied Physiology
    PubMed ID:
    26542520