RayBio® C-Series Mouse Cytokine Antibody Array 4 Kit. Detects 34 Mouse Cytokines. Suitable for all liquid sample types.
Mouse Cytokine Array C4
Mouse Cytokine Array C4
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 34 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
bFGF
| CD26 (DPPIV)
| Dtk
| E-Selectin
| Fc gamma RIIB (CD32b)
|
Flt-3 Ligand
| GITR (TNFRSF18)
| HGFR
| ICAM-1 (CD54)
| IGFBP-2
|
IGF-1
| IGF-2
| IL-15
| IL-17 RB
| IL-7
|
I-TAC (CXCL11)
| Lungkine (CXCL15)
| MDC (CCL22)
| MMP-2
| MMP-3
|
Osteopontin (SPP1)
| Osteoprotegerin (TNFRSF11B)
| Pro-MMP-9
| Resistin
| Sonic Hedgehog N-Terminal (Shh-N)
|
TCK-1 (CXCL7)
| TIMP-2
| TRANCE (TNFSF11)
| TROY (TNFRSF19)
| TSLP
|
VEGFR1
| VEGFR2
| VEGFR3
| VEGF-D
| |
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array C4 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Oncogene inactivation-induced senescence facilitates tumor relapse
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (conditioned medium CM from proliferating, senescent clone 4 cells)
- Year:
- 2026
- Journal Name:
- Nature Communications
- PubMed ID:
- 42457681
KITENIN-CCL20 axis is a potential therapeutic target for modulating immunosuppressive tumor microenvironment in glioblastoma
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (supernatant from KITENIN-modulated GL261 cells)
- Year:
- 2026
- Journal Name:
- Neurotherapeutics
- PubMed ID:
- 41688273
The cost of persistent alarm: temporal transcriptional reprogramming of macrophages from acute activation to chronic immune suppression by day 11
- Year:
- 2026
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 42643373
Signatures in the Protein Content of Human and Murine Blood Serum Exosomes, in the Context of Major Depressive Disorder, Are Associated with Cytokine Activity
- Species:
- Mouse
- Sample Type:
- Serum (lysed exosome-enriched EV samples isolated from mouse serum); Extracellular Vesicles
- Year:
- 2026
- Journal Name:
- Cells
- PubMed ID:
- 42346072
NEMO/NF-κB signaling functions as a double-edged sword in PanIN formation versus progression to pancreatic cancer
- Year:
- 2024
- Journal Name:
- Molecular Cancer
- PubMed ID:
- 38755681
IL1R2 Blockade Alleviates Immunosuppression and Potentiates Anti-PD-1 Efficacy in Triple-Negative Breast Cancer
- Year:
- 2024
- Journal Name:
- Cancer Research
- PubMed ID:
- 38657120
Tumor-associated neutrophils upregulate Nectin2 expression, creating the immunosuppressive microenvironment in pancreatic ductal adenocarcinoma
- Year:
- 2024
- Journal Name:
- Journal of Experimental & Clinical Cancer Research
- PubMed ID:
- 39261943
Systemic versus local delivery of mesenchymal stem cells to improve the early stages of fracture healing in a polytrauma model
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (conditioned media from MSCs)
- Year:
- 2025
- Journal Name:
- Journal of Biological Engineering
- PubMed ID:
- 41029699
High-Dose Tramadol Enhances the Proliferative and Invasive Potential of Pancreatic Ductal Adenocarcinoma in Mice Through Microenvironmental Alteration
- Species:
- Mouse
- Sample Type:
- Plasma (pooled plasma)
- Year:
- 2026
- Journal Name:
- Pain research & management
- PubMed ID:
- 42325112
Loss of cPLA2α function attenuates inflammation and epithelial thickening in a mouse model of Haemophilus influenzae-mediated COPD exacerbation
- Species:
- Mouse
- Sample Type:
- Bronchoalveolar Lavage (cell-free BAL fluid pools); Other Body Fluid
- Year:
- 2026
- Journal Name:
- Current research in microbial sciences
- PubMed ID:
- 41675617