Mouse Chemokine Array Q1 (Glass Slide)

Referencia QAM-CHE-1-2

embalaje : 22SampleKit

Marca : RayBiotech

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Mouse Chemokine Array Q1

Quantibody® Mouse Chemokine Array 1 Kit. Detects 25 Mouse Chemokines. Suitable for all liquid sample types.

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Product Description

Specifications

Size8 Sample Kit, 22 Sample Kit, 50 Sample Kit
SpeciesMouse
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeQuantitative
Number of Targets Detected25
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Research AreaInflammation, Chemokine Biology
Shipping TypeBlue ice
Storage-20°C

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
6Ckine
(CCL21)
  • Gene Symbol: CCL21
  • Accession #: P86792
  • ELISA Kit: RayBio Mouse 6Ckine ELISA
BLC
(CXCL13)
  • Gene Symbol: CXCL13
  • Accession #: O55038
  • ELISA Kit: RayBio Mouse BLC ELISA
CTACK
(CCL27)
  • Gene Symbol: CCL27
  • Accession #: Q9Z1X0
  • ELISA Kit: RayBio Mouse CTACK ELISA
CXCL16
  • Gene Symbol: CXCL16
  • Accession #: Q5F2D5
  • ELISA Kit: RayBio Mouse CXCL16 ELISA
Eotaxin-1
(CCL11)
  • Gene Symbol: CCL11
  • Accession #: P48298
  • ELISA Kit: RayBio Mouse Eotaxin-1 ELISA
Eotaxin-2
(MPIF-2/CCL24)
  • Gene Symbol: Ccl24
  • Accession #: Q9JKC0
  • ELISA Kit: RayBio Mouse Eotaxin-2 ELISA
Fractalkine
(CX3CL1)
  • Gene Symbol: CX3CL1
  • Accession #: O35188
  • ELISA Kit: RayBio Mouse Fractalkine ELISA
I-309
(TCA-3/CCL1)
  • Gene Symbol: CCL1
  • Accession #: P14098
  • ELISA Kit: RayBio Mouse TCA-3 ELISA
I-TAC
(CXCL11)
  • Gene Symbol: CXCL11
  • Accession #: Q9JHH5
  • ELISA Kit: RayBio Mouse I-TAC ELISA
KC
(CXCL1)
  • Gene Symbol: CXCL1
  • Accession #: P12850
  • ELISA Kit: RayBio Mouse KC ELISA
LIX
  • Gene Symbol: Cxcl5
  • Accession #: P50228
  • ELISA Kit: RayBio Mouse LIX ELISA
MCP-1
(CCL2)
  • Gene Symbol: CCL2
  • Accession #: Q9QYD7
  • ELISA Kit: RayBio Mouse MCP-1 ELISA
MCP-5
  • Gene Symbol: Ccl12
  • Accession #: Q9QYD6
  • ELISA Kit: RayBio Mouse MCP-5 ELISA
MDC
(CCL22)
  • Gene Symbol: CCL22
  • Accession #: O88430
  • ELISA Kit: RayBio Mouse MDC ELISA
MIG
(CXCL9)
  • Gene Symbol: CXCL9
  • Accession #: P18340
  • ELISA Kit: RayBio Mouse MIG ELISA
MIP-1 alpha
(CCL3)
  • Gene Symbol: CCL3
  • Accession #: P14096
  • ELISA Kit: RayBio Mouse MIP-1 alpha ELISA
MIP-1 gamma
  • Gene Symbol: Ccl9
  • Accession #: Q5QNW2
  • ELISA Kit: RayBio Mouse MIP-1 gamma ELISA
MIP-2
  • Gene Symbol: Cxcl2
  • Accession #: P10889
  • ELISA Kit: RayBio Mouse MIP-2 ELISA
MIP-3 alpha
(CCL20)
  • Gene Symbol: CCL20
  • Accession #: O89093
  • ELISA Kit: RayBio Mouse MIP-3 alpha ELISA
MIP-3 beta
(CCL19)
  • Gene Symbol: CCL19
  • Accession #: O70460
  • ELISA Kit: RayBio Mouse MIP-3 beta ELISA
Platelet Factor 4
(CXCL4)
  • Gene Symbol: PF4, CXCL4, SCYB4
  • Accession #: Q9Z126
  • ELISA Kit: RayBio Mouse PF-4 ELISA
RANTES
(CCL5)
  • Gene Symbol: CCL5
  • Accession #: P30882
  • ELISA Kit: RayBio Mouse RANTES ELISA
SDF-1 alpha
(CXCL12 alpha)
  • Gene Symbol: CXCL12
  • Accession #: P40224
  • ELISA Kit: RayBio Mouse SDF-1 alpha ELISA
TARC
(CCL17)
  • Gene Symbol: CCL17
  • Accession #: Q9WUZ6
  • ELISA Kit: RayBio Mouse TARC ELISA
TECK
(CCL25)
  • Gene Symbol: CCL25
  • Accession #: O35903
  • ELISA Kit: RayBio Mouse TECK ELISA

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Mouse Chemokine Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAM-CHE-1.gal)
Excel (QAM-CHE-1_Analysis_Tool.xls)

citations

  • Histone deacetylase inhibition induces an antitumor IRF8-dependent trained immunity-like status in macrophages

    Species:
    Mouse
    Sample Type:
    Cell Culture Supernatant (macrophage supernatants)
    Year:
    2026
    Journal Name:
    Nature Communications
  • CD34+ PI16+ fibroblast progenitors aggravate neointimal lesions of allograft arteries via CCL11/CCR3-PI3K/AKT pathway

    Species:
    Mouse
    Sample Type:
    Cell Culture Supernatant (cell supernatant from CD34-positive, CD34-negative cells)
    Year:
    2025
    Journal Name:
    Theranostics
    PubMed ID:
    39990233
  • Targeting IL-17A enhances imatinib efficacy in Philadelphia chromosome-positive B-cell acute lymphoblastic leukemia

    Species:
    Mouse
    Sample Type:
    Serum (WT and BCR-ABLtTA mice)
    Year:
    2024
    Journal Name:
    Nature Communications
    PubMed ID:
    38172124
  • Fetal-derived PF4+ tissue-resident macrophages recruit neutrophils as a key mechanism underlying DENV-2-induced intrauterine growth restriction in mice

    Species:
    Mouse
    Sample Type:
    Other / Unspecified (placenta)
    Year:
    2026
    Journal Name:
    Plos Pathogens
    PubMed ID:
    42640962
  • Sphingosine Kinase 1 Aggravates Liver Fibrosis by Mediating Macrophage Recruitment and Polarization

    Species:
    Mouse
    Sample Type:
    Tissue Lysate / Homogenate (liver tissue homogenates)
    Year:
    2024
    Journal Name:
    Cellular and Molecular Gastroenterology and Hepatology
    PubMed ID:
    39305988
  • Optimized CAR-T therapy based on spatiotemporal changes and chemotactic mechanisms of MDSCs induced by hypofractionated radiotherapy

    Species:
    Mouse
    Sample Type:
    CT26 cell-Xenograft, serum
    Year:
    2023
    Journal Name:
    Molecular Therapy
  • Huiyang Shengji decoction promotes wound healing in diabetic mice by activating the EGFR/PI3K/ATK pathway

    Species:
    Mouse
    Sample Type:
    Tissue Lysate / Homogenate (wound skin tissue lysates)
    Year:
    2021
    Journal Name:
    Chinese Medicine
    PubMed ID:
    34727961
  • Airborne lipid antigens mobilize resident intravascular NKT cells to induce allergic airway inflammation

    Species:
    Mouse
    Sample Type:
    Tissue Lysate (Lung Tissue Lysate)
    Year:
    2011
    Journal Name:
    Journal of Experimental Medicine
  • Vibsanin B preferentially targets HSP90, inhibits interstitial leukocyte migration, and ameliorates experimental autoimmune encephalomyelitis

    Species:
    Mouse
    Sample Type:
    Tissue Lysate (Mouse Spinal cords during encephalomyelitis)
    Year:
    2015
    Journal Name:
    Journal of Immunology
    PubMed ID:
    25810397
  • Protective Role of the Inflammatory CCR2/CCL2 Chemokine Pathway through Recruitment of Type 1 Cytotoxic T Lymphocytes to Tumor Beds

    Species:
    Mouse
    Sample Type:
    Tissue Lysate (Tumor lysate from B16-F0 cells)
    Year:
    2013
    Journal Name:
    Journal of Immunology

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