Mouse CD54/ICAM-1 Antibody : APC
Referencia OASB00496
embalaje : 0.1mg
Marca : Aviva Systems Biology
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Teléfono : +1 850 650 7790
Mouse CD54/ICAM-1 Antibody : APC (OASB00496)
| Datasheets/Manuals | Printable datasheet for Mouse CD54/ICAM-1 Antibody : APC (OASB00496) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | YN1/1.7.4 |
| Isotype | IgG2b |
| Host | Rat |
| Conjugation | APC |
| Application | FC |
| Additional Information | Description: CD54, a member of the immunoglobulin superfamily of cell surface receptors and also known as Intercellular Adhesion Molecule-1 (ICAM-1), is a type I transmembrane glycoprotein that mediates important cell/cell interactions. CD54 is expressed on endothelial cells, dendritic cells, keratinocytes, and lymphocytes. Its expression is upregulated by inflammatory mediators such as IFN-gamma, IL-1, TNF-alpha, lipopolysaccharide, and phorbol esters. Endothelial CD54 contributes to the extravasation of leukocytes from blood vessels, particularly in areas of inflammation. CD54 on antigen-presenting cells contributes to antigen-specific T-cell activation, presumably by enhancing interactions between T cell and antigen-presenting cells. These adhesion reactions are mediated via the binding of CD54 to its major ligands, LFA-1 and Mac-1. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Mouse NS-1 cells |
| Concentration | 0.1 mg/mL |
| Specificity | CD54 |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. |
| Dilution | Flow Cytometry: Fluorescein conjugate Biotin conjugate PE and APC conjugates <=1 ug/106 cells <=1 ug/106 cells <= 0.2 ug/106 cells |
| Application Info | Flow cytometry 1,2, Immunohistochemistry (frozen sections) 4, Immunoprecipitation 1, In vitro blocking studies 1, Western blotting 3 |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and Allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Takei, F. 1985. J. Immunol. 134:1403 2. Horley, K.J., C. Carpenito, B. Baker, F. Takei. 1989. EMBO. J. 8:2889 3. Welder, Clayton A., D.H.S. Lee, F. Takei. 1993. J. Immunol. 150:2203 4. Qin, Lan, et. al. 1996. J. Immunol. 157:5016. |
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| Gene Symbol | ICAM1 |
| Gene Full Name | Intercellular adhesion molecule 1 |
| Alias Symbols | MAL, CD54, Icam, Ly-4, Ly-47, Icam-1, MALA-2 |
| NCBI Gene Id | 15894; 396750 |
| Protein Name | intercellular adhesion molecule 1 |
| Description of Target | This gene encodes an integral membrane protein that binds leukocyte adhesion protein LFA-1. It participates in the innate immune response. |
| Uniprot ID | P13597, Q9MZU5 |
| Protein Accession # | NP_034623.1 |
| Nucleotide Accession # | NM_010493.3 |
