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> mouse Anti-IgE, Heavy Chain (MaxLight 550) Monoclonal Antibody [Clone:3H2277 (MARE-1)]

mouse Anti-IgE, Heavy Chain (MaxLight 550) Monoclonal Antibody [Clone:3H2277 (MARE-1)]

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Referencia I1900-65-ML550-100ul

embalaje : 100ul

Marca : US Biological

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Teléfono : +1 850 650 7790

I1900-65-ML550 IgE, Heavy Chain (MaxLight 550)

Clone Type
Monoclonal
Host
mouse
Source
rat
Swiss Prot
P01855
Isotype
IgG1
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Applications:
Suitable for use in FLISA and Western Blot. Other applications have not been tested

Recommended Dilutions:
Optimal working dilutions to be determined by researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice fused with mouse SP2/0 myeloma cells

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2277 (MARE-1)|Host: mouse|Source: rat|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.|Specificity: Recognizes the epsilon chain of rat IgE. Does not cross-react with other classes of rat immunoglobulin. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the epsilon chain of rat IgE. Does not cross-react with other classes of rat immunoglobulin.
References
1. Bazin H, et al. 1987. J. Immunol. 121: 2077-2081. 2. Bazin H, et al. 1984. J. Imm. Methods. 71: 9-16. 3. Bazin H, et al. 1984. J. Imm. Methods. 66: 261-269.