Mouse Anti-CD31 Monoclonal Antibody (clone: 158-2B3) Pacific Blue conjugated

Description：Mouse monoclonal antibody Pacific Blue labled to CD31Immunogen：Native purified human CD31.Purification：The antibody was purified by affinity chromatography.Clonality：Monoclonal (clone: 158-2B3)Conjugation：Pacific BlueForm：Mouse IgG1. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.Dilution：10 μl / assayGene Symbol：PECAM1Alternative Names：Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31 Entrez Gene (Human)： 5175; SwissProt (Human)： P16284; Directions for Use：1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature. 7. Repeat step 5. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).Storage/Stability：Shipped and store at 4°C for one year. Do not freeze.