MCL1 ELISA Kit (Human)
Referencia OKCD08205
embalaje : 96Wells
Marca : Aviva Systems Biology
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Teléfono : +1 850 650 7790
MCL1 ELISA Kit (Human) (OKCD08205)
| Datasheets/Manuals | Printable datasheet for MCL1 ELISA Kit (Human) (OKCD08205) |
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| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | < 0.127ng/mL |
| Gene Symbol | MCL1 |
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| Gene Full Name | MCL1 apoptosis regulator, BCL2 family member |
| Alias Symbols | BCL2 family apoptosis regulator;bcl2-L-3;BCL2L3;bcl-2-like protein 3;bcl-2-related protein EAT/mcl1;EAT;induced myeloid leukemia cell differentiation protein Mcl-1;Mcl-1;MCL1, BCL2 family apoptosis regulator;mcl1/EAT;MCL1-ES;MCL1L;MCL1S;myeloid cell leukemia 1;myeloid cell leukemia ES;myeloid cell leukemia sequence 1 (BCL2-related);TM. |
| NCBI Gene Id | 4170 |
| Protein Name | Induced myeloid leukemia cell differentiation protein Mcl-1 |
| Description of Target | Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. |
| Uniprot ID | Q07820 |
| Protein Accession # | NP_001184249.1 |
| Nucleotide Accession # | NM_001197320.1 |
