LSD1, Chemiluminescent, BioAssay™ Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)

LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development.

Intended Use:
The LSD1 Chemiluminescent BioAssay™ Kit is designed to directly measure activity of human lysine-specific demethylase (LSD1) for screening and profiling applications.

Test Principle:
The key to the LSD1 Chemiluminescent BioAssay™ Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing LSD1 enzyme is incubated with a sample containing assay buffer. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt

Applications:
Great for studying enzyme kinetics and HTS applications.

Kit Components:
Microplate precoated with histone substrate, 1x plate
LSD1, 1x10ug
Primary antibody 10, 1x12.5ul
Secondary HRP-labeled antibody 1, 1x10ul
3x LSD1 assay buffer 2, 1x3ml
Blocking buffer, 1x50ml
HRP chemiluminescent substrate A, (transparent bottle), 1x6ml
HRP chemiluminescent substrate B, (brown bottle), 1x6ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.