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> LKB1 (STK11) (NM_000455) Human Over-expression Lysate

LKB1 (STK11) (NM_000455) Human Over-expression Lysate

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Referencia LY424705

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LKB1 (STK11) (NM_000455) Human Over-expression Lysate

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SKU
LY424705
Transient overexpression lysate of serine/threonine kinase 11 (STK11) (as WB positive control)
 

Other products for "LKB1 or NM_000455"

Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202885
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000455, NP_000446
Synonyms hLKB1; LKB1; PJS
Predicted MW 48.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000446
Locus ID 6794
Cytogenetics 19p13.3
Protein Families WB
Protein Pathways Adipocytokine signaling pathway, mTOR signaling pathway
Product Manuals
  • Western Blot Protocols
FAQs
  • Over-expression Cell Lysate
SDS
SKU Description Size
LC424705 Lyophilized STK11 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
TP761326 Purified recombinant protein of Human serine/threonine kinase 11 (STK11), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug 50 ug

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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