Lipo2K Transfection Reagent is a cationic lipid transfection reagent widely used in cell transfection, which can transfect DNA or RNA (such as siRNA/mRNA) into various types of cells, including some difficult-to-transfect cells. The transfection reagent is used in the same way as the commonly used Lipofectamine 2000 Reagent, and the transfection efficiency of this transfection reagent has been tested to be comparable to or slightly higher than the Lipofectamine® 2000 Reagent.

This transfection reagent has the advantages of high transfection efficiency, simple operation, good reproducibility and no obvious cytotoxicity, and is suitable for transfection of adherent cells and suspension cells. In addition, this transfection reagent is not only suitable for transfection of a single plasmid or siRNA, but also for transfection of multiple plasmids or co-transfection of plasmids and siRNAs. Plasmid expression can be detectable 24-48 h after transfection, and siRNA silencing should be achieved 3-5 days after transfection.

When transfecting cells with this reagent, the presence of serum or antibiotics does not affect the transfection efficiency. However, for optimal transfection, it is recommended to use a serum-containing medium without antibiotics for transfection.

Figure 1: Lipo2K Transfection Reagent (Left) compared to Lipofectamine® 2000 Reagent (Right) transfected HEK293T cells