Specifications

Product Data
Type	Human Untagged Clone
Target Symbol	LGI4
Synonyms	AMC1; AMCNMY; LGIL3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Restriction Sites	SgfI-MluI |
ACCN	NM_139284
Insert Size	1614 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping	Ambient
Reference Data
RefSeq	NM_139284.2
RefSeq Size	2906 bp
RefSeq ORF	1614 bp
Locus ID	163175
UniProt ID	Q8N135
Cytogenetics	19q13.11
Domains	EPTP, LRR, LRRCT, LRR_TYP
MW	59.1 kDa
Summary	Component of Schwann cell signaling pathway(s) that controls axon segregation and myelin formation (By similarity).UniProtKB/Swiss-Prot Function

Product Manuals

TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System TrueClones: Full length cDNA clones (Human/Mouse/Rat), expressing native proteins pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits

FAQs

TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent

SDS

SDS for SC313909

cDNA Clone Resources

Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones Protocol: Lentivirus packaging Product Sheet: AAV AAV Biosafety Lentivirus and AAV FAQ Virus-Mediated Gene Delivery

SKU	Description	Size
RC219293	LGI4 (Myc-DDK-tagged)-Human leucine-rich repeat LGI family, member 4 (LGI4)	10 ug
RC219293L3	Lenti ORF clone of Human leucine-rich repeat LGI family, member 4 (LGI4), Myc-DDK-tagged	10 ug
RC219293L4	Lenti ORF clone of Human leucine-rich repeat LGI family, member 4 (LGI4), mGFP tagged	10 ug
RG219293	LGI4 (tGFP-tagged) - Human leucine-rich repeat LGI family, member 4 (LGI4)	10 ug