Lens culinaris (LCA/LCH)

Fractionated Lens culinaris lectin (LCA) is composed of four subunits that consists of an alpha-chain (MW: 5.7kD) and β-chain (MW: 17.5kD). It has a carbohydrate specificity toward alpha-D-mannose and alpha-D-glucose, eluting with the sugar MealphaMan+MealphaGlc. This lectin is useful in affinity chromatography columns for the separation of glycoconjugates. The two isomers LCA-A and LCA-B agglutinates human red blood cells, although not blood group specific. Activity of LCA is determined by haemagglutination with human blood and agglutinates a 2% suspension of human erythrocytes at a lectin concentration of 8µg/ml. LCA requires calcium and manganese ions for binding. See other LCA conjugates.

Source:
Lentil

Blood Specificity:
Non-specific

Sugar Specificity:
alpha-Mannose, alpha-Glucose

Inhibitory Carbohydrate:
alpha-Methylmannoside + alpha-Methylglucoside

Divalent Ions:
Ca++, Mn++

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.