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> Lactic acid [50-21-5]

Lactic acid [50-21-5]

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Referencia HY-B2227-100mg

embalaje : 100mg

Marca : MedChemExpress

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Descripciòn

Lactic acid (DL-Lactic acid) is a hydroxycarboxylic acid receptor 1 (HCAR1) activator and an epigenetic modulator inducing lysine residues lactylation. Lactic acid is a glycolysis end-product, bridging the gap between glycolysis and oxidative phosphorylation. Lactic acid is an oncometabolite and has immune protective role of lactate in anti-tumor immunity[1][2]. Lactic acid also has antimicrobial activity, which can be used as a food preservative[3].

IC50 & Target[1][2]

HCAR1

 

Human Endogenous Metabolite

 

Cellular Effect
Cell Line Type Value Description References
A549 IC50
26.6 μM
Compound: Lactic acid
Antiproliferative activity against human A549 cells after 48 hrs by MTT assay
Antiproliferative activity against human A549 cells after 48 hrs by MTT assay
[PMID: 28710964]
A549 IC50
26.61 mM
Compound: Lactic acid
Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
[PMID: 25299679]
MRC5 IC50
23.93 mM
Compound: Lactic acid
Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 48 hrs by MTT assay
[PMID: 25299679]
MRC5 IC50
23.93 μM
Compound: Lactic acid
Cytotoxicity against human MRC5 cells after 48 hrs by MTT assay
Cytotoxicity against human MRC5 cells after 48 hrs by MTT assay
[PMID: 28710964]
In Vitro

Lactic acid (20 mM; 24, 48 h) results in a significant decrease in cell proliferation and migration, acting and switching cell metabolism toward oxidative phosphorylation[1].
Lactic acid (20 mM; 24 h) shows an increase in the protein and mRNA expression levels of MCT1 and HCAR1[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lactic acid Related Antibodies

Cell Proliferation Assay[1]

Cell Line: Uveal melanoma 92.1 cells
Concentration: 20 mM
Incubation Time: 24, 48 h
Result: Inhibited cell proliferation and migration.

Western Blot Analysis[1]

Cell Line: Uveal melanoma cells
Concentration: 20 mM
Incubation Time: 24 h
Result: Showed an increase in the protein expression levels of MCT1 and HCAR1

RT-PCR[1]

Cell Line: Uveal melanoma cells
Concentration: 20 mM
Incubation Time: 24 h
Result: Induced a significant increase in mRNA expression levels of SLC16A1 and HCAR1.
In Vivo

Lactic acid (1.6 g/kg; pH 7.4; subcutaneous; daily from Day 8 to 30 days) shows CD8+ T cell-dependent tumor growth inhibition in MC38 colon cancer model[2].
Note: The pH of the working solution needs to be adjusted to around 7.4 before administration to animals.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female C57BL/6 mice with MC38 colon cancer cells[2]
Dosage: 1.6 g/kg
Administration: Subcutaneous; daily from Day 8 to 30 days
Result: Significantly suppressed tumor growth.
Peso molecular

90.08

Fòrmula

C3H6O3
No. CAS
Appearance

Liquid (Density: 1.209 g/cm3)

Color

Colorless to light yellow

SMILES

CC(O)C(O)=O
Structure Classification
Initial Source
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvente y solubilidad
In Vitro: 

H2O : ≥ 200 mg/mL (2220.25 mM)

DMSO : ≥ 200 mg/mL (2220.25 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 11.1012 mL 55.5062 mL 111.0124 mL
5 mM 2.2202 mL 11.1012 mL 22.2025 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (55.51 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL (55.51 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureza y Documentación
Referencias

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