IL16 ELISA Kit (Rabbit)

Referencia OKWB00425

embalaje : 96Wells

Marca : Aviva Systems Biology

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Teléfono : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for IL16 ELISA Kit (Rabbit) (OKWB00425)
Product Info
Predicted Species ReactivityOryctolagus cuniculus|Rabbit
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)88-105%85-104%85-95%87-105%
EDTA Plasma (n=5)90-98%82-100%94-101%93-100%
Heparin Plasma (n=5)89-97%84-99%82-98%81-94%
ELISA Kit PrincipleAviva Systems Biology IL16 ELISA Kit (Rabbit) (OKWB00425) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL16 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL16 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL16 captured in the well.
ELISA Kit Range15.625-1000pg/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)85-10391
EDTA Plasma (n=5)90-10297
Heparin Plasma (n=5)92-9896
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
IL16 Microplate96 Wells (12 x 8 Well strips)
IL16 Lyophilized Standard2 x 1 ng
100X IL16 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity9.4 pg/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolIL16
Protein NamePro-interleukin-16
Description of TargetInterleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
Uniprot IDG1SZ67