IgG4-S228P L235E P329G (SPLEPG) Isotype Control, In Vivo Grade Recombinant Human

The ideal recombinant human IgG4 isotype control antibody (hIgG4 S228P kappa isotype control) has low or no specific binding to any human sample, and humanized or completely human variable regions in addition to the human IgG4 S228P kappa constant regions. The human IgG4 kappa isotype control and mutants can be used in the cell-based assay or animal model as the antibody negative control when evaluating fully human, humanized, or chimeric antibodies.

The hIgG4 isotype control mutants were generated because the corresponding mutations had been introduced to some human IgG4 therapeutic antibody candidates. For example, S228P, an engineered mutation in the core hinge region, is used to prevent Fab-arm exchange in vivo of human IgG4 molecules; P329G substitution combined with S228P and L235E was shown to decrease effector functions by disrupting the Fc/Fcγ receptor interface. When clients test their engineered antibody candidates, the silent Fc effctor function human IgG4 isotype controls are useful for in vivo use.

Applications:
Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry and in vivo animal model research. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: frozen/paraffin
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.